The continued corona virus illness 2019 (COVID-19) pandemic, brought on by SARS-CoV-2 an infection, has resulted in a whole bunch of hundreds of deaths. Mobile entry of SARS-CoV-2, which is mediated by the viral spike protein and ACE2 receptor, is a necessary goal for the event of vaccines, therapeutic antibodies, and medicines. Utilizing a mammalian cell expression system, a genetically engineered sensor of fluorescent protein (Gamillus)-fused SARS-CoV-2 spike trimer (STG) to probe the viral entry course of is developed.
In ACE2-expressing cells, it’s discovered that the STG probe has glorious efficiency within the live-cell visualization of receptor binding, mobile uptake, and intracellular trafficking of SARS-CoV-2 underneath virus-free situations. The brand new system permits quantitative analyses of the inhibition potentials and detailed affect of COVID-19-convalescent human plasmas, neutralizing antibodies and compounds, offering a flexible software for high-throughput screening and phenotypic characterization of SARS-CoV-2 entry inhibitors. This strategy might also be tailored to develop a viral entry visualization system for different viruses.
Clinically amyopathic dermatomyositis (CADM) is a uncommon, aggressive variant of dermatomyositis related to interstitial lung illness (ILD) and refractoriness to immunosuppressants. Antibodies towards melanoma differentiation-associated gene 5 (MDA-5) are sometimes present in sufferers with CADM. We report a affected person with superior CADM with ILD and MDA-5 antibodies who failed to enhance with immunosuppressants. We carried out 2 TPE over three days, utilizing 5% albumin as alternative fluid.
Though 5 complete TPE had been deliberate, he was transferred for lung transplant analysis after the second TPE; he died 16 days after switch with out receiving a transplant. A literature assessment recognized 4 sufferers with CADM and MDA-5 antibodies handled with TPE; all skilled symptomatic enchancment of their ILD. We attribute our affected person’s final result to the superior nature of his illness quite than a failure of TPE. Further analysis could point out a attainable reclassification of CADM with MDA-5 antibodies in future ASFA tips.
Focused antigen supply to cross-presenting dendritic cells (DC) in vivo effectively induces T effector cell responses and shows a beneficial strategy in vaccine design. Antigen is delivered to DC by way of antibodies particular for endocytosis receptors corresponding to DEC-205 that induce uptake, processing, and MHC class I- and II-presentation. Environment friendly and dependable conjugation of the specified antigen to an acceptable antibody is a essential step in DC concentrating on and amongst different components is determined by the format of the antigen. Chemical conjugation of full-length protein to purified antibodies is one attainable technique. Prior to now, we have now efficiently established cross-linking of the mannequin antigen ovalbumin (OVA) and a DEC-205-specific IgG2a antibody (αDEC-205) for in vivo DC concentrating on research in mice.
Step one of the protocol is the purification of the antibody from the supernatant of the NLDC (non-lymphoid dendritic cells)-145 hybridoma by affinity chromatography. The purified antibody is activated for chemical conjugation by sulfo-SMCC (sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate) whereas on the similar time the sulfhydryl-groups of the OVA protein are uncovered via incubation with TCEP-HCl (tris (2-carboxyethyl) phosphine hydrochloride).
Extra TCEP-HCl and sulfo-SMCC are eliminated and the antigen is combined with the activated antibody for in a single day coupling. The ensuing αDEC-205/OVA conjugate is concentrated and free of unbound OVA. Profitable conjugation of OVA to αDEC-205 is verified by western blot evaluation and enzyme-linked immunosorbent assay (ELISA). We’ve got efficiently used chemically crosslinked αDEC-205/OVA to induce cytotoxic T cell responses within the liver and to check completely different adjuvants for his or her potential in inducing humoral and mobile immunity following in vivo concentrating on of DEC-205+ DC.
Past that, such chemically coupled antibody/antigen conjugates provide beneficial instruments for the environment friendly induction of vaccine responses to tumor antigens and have been confirmed to be superior to classical immunization approaches relating to the prevention and remedy of assorted varieties of tumors.
Human fascioliasis is a public well being drawback significantly in areas the place ruminants are raised. The goals of this research had been to find out the seroprevalence of anti-Fasciola antibody and the related danger components amongst cattle farm staff and dwellers in Kelantan.
A complete of 90 blood samples had been collected on this cross-sectional research. A set of validated questionnaire was used to acquire data on socio-demographic profiles and dietary habits of members. The sera had been subjected to enzyme linked immunosorbent assay (ELISA) for the detection of anti-Fasciola IgG antibody. The affiliation between seropositivity and the numerous danger components had been decided by way of logistic regression. From the consequence, serological screening revealed 60 (67%) members optimistic for anti-Fasciola IgG antibody.
PRDM16 Polyclonal Antibody |
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A-2016 | EpiGentek |
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PRDM17 Polyclonal Antibody |
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A-2017 | EpiGentek |
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EZH1 Polyclonal Antibody |
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A-2018 | EpiGentek |
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HSF1 Polyclonal Antibody |
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A-2401 | EpiGentek |
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PRMT3 Polyclonal Antibody |
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A-3003 | EpiGentek |
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SET1 Polyclonal Antibody |
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A-3011 | EpiGentek |
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SET07 Polyclonal Antibody |
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A-3013 | EpiGentek |
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LSD1 Polyclonal Antibody |
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A-3018 | EpiGentek |
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HDAC10 Polyclonal Antibody |
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A-4010 | EpiGentek |
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PCAF Polyclonal Antibody |
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A-4012 | EpiGentek |
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Histone H3K9ac (Acetyl H3K9) Polyclonal Antibody |
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A-4022 | EpiGentek |
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Histone H3K14ac (Acetyl H3K14) Polyclonal Antibody |
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A-4023 | EpiGentek |
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Histone H3K18ac (Acetyl H3K18) Polyclonal Antibody |
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A-4024 | EpiGentek |
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Histone H3K23ac (Acetyl H3K23) Polyclonal Antibody |
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A-4025 | EpiGentek |
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Histone H3K56ac (Acetyl H3K56) Polyclonal Antibody |
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A-4026 | EpiGentek |
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Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody |
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A-4031 | EpiGentek |
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Histone H3K4me2 (H3K4 Dimethyl) Polyclonal Antibody |
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A-4032 | EpiGentek |
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Histone H3K4me3 (H3K4 Trimethyl) Polyclonal Antibody |
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A-4033 | EpiGentek |
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Histone H3K9me1 (H3K9 Monomethyl) Polyclonal Antibody |
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A-4034 | EpiGentek |
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Histone H3K9me2 (H3K9 Dimethyl) Polyclonal Antibody |
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A-4035 | EpiGentek |
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Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody |
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A-4036 | EpiGentek |
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Histone H3K27me1 (H3K27 Monomethyl) Polyclonal Antibody |
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A-4037 | EpiGentek |
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Histone H3K27me2 (H3K27 Dimethyl) Polyclonal Antibody |
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A-4038 | EpiGentek |
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Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody |
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A-4039 | EpiGentek |
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Histone H3K36me1 (H3K36 Monomethyl) Polyclonal Antibody |
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A-4040 | EpiGentek |
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Histone H3K36me2 (H3K36 Dimethyl) Polyclonal Antibody |
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A-4041 | EpiGentek |
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Histone H3K36me3 (H3K36 Trimethyl) Polyclonal Antibody |
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A-4042 | EpiGentek |
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Histone H3K79me1 (H3K79 Monomethyl) Polyclonal Antibody |
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A-4043 | EpiGentek |
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Histone H3K79me2 (H3K79 Dimethyl) Polyclonal Antibody |
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A-4044 | EpiGentek |
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Histone H3K79me3 (H3K79 Trimethyl) Polyclonal Antibody |
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A-4045 | EpiGentek |
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Histone H4K20me1 (H4K20 Monomethyl) Polyclonal Antibody |
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A-4046 | EpiGentek |
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Histone H4K20me2 (H4K20 Dimethyl) Polyclonal Antibody |
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A-4047 | EpiGentek |
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Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody |
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A-4048 | EpiGentek |
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Histone H3K27ac (Acetyl H3K27) Polyclonal Antibody |
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A-4708 | EpiGentek |
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CRISPR Cas9 Monoclonal Antibody [7A9] |
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A-9000 | EpiGentek |
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CRISPR/Cas9 (SaCas9) Monoclonal Antibody [6H4] |
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A-9001 | EpiGentek |
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CLIMP-63 Polyclonal Antibody |
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A-0701 | EpiGentek |
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DNMT3A Polyclonal Antibody |
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A-1003 | EpiGentek |
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DNMT3B Polyclonal Antibody |
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A-1004 | EpiGentek |
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DNMT3L Polyclonal Antibody |
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A-1005 | EpiGentek |
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MBD3 Polyclonal Antibody |
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A-1008 | EpiGentek |
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MGMT Polyclonal Antibody |
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A-1010 | EpiGentek |
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MeCP2 Polyclonal Antibody |
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A-1012 | EpiGentek |
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TET1 Polyclonal Antibody |
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A-1020 | EpiGentek |
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DNMT1 Polyclonal Antibody |
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A-1700 | EpiGentek |
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TET2 Polyclonal Antibody |
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A-1701 | EpiGentek |
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MBD2 Polyclonal Antibody |
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A-1713 | EpiGentek |
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PRDM2 Polyclonal Antibody |
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A-2002 | EpiGentek |
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PRDM3 Polyclonal Antibody |
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A-2003 | EpiGentek |
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PRDM6 Polyclonal Antibody |
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A-2006 | EpiGentek |
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PRDM12 Polyclonal Antibody |
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A-2012 | EpiGentek |
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PRDM14 Polyclonal Antibody |
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A-2014 | EpiGentek |
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EZH2 Polyclonal Antibody |
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A-2019 | EpiGentek |
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EED Polyclonal Antibody |
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A-2020 | EpiGentek |
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Swi2/SNF2 Polyclonal Antibody |
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A-2023 | EpiGentek |
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SNFa/BRM Polyclonal Antibody |
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A-2025 | EpiGentek |
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Ini1 Polyclonal Antibody |
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A-2026 | EpiGentek |
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RNA Polymerase II Monoclonal Antibody [CTD4H8] |
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A-2032 | EpiGentek |
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CBX5 Polyclonal Antibody |
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A-2701 | EpiGentek |
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EGLN1 Polyclonal Antibody |
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A-2702 | EpiGentek |
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RBBP4 Polyclonal Antibody |
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A-2703 | EpiGentek |
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SIN3A Polyclonal Antibody |
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A-2704 | EpiGentek |
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CTBP1 Polyclonal Antibody |
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A-2705 | EpiGentek |
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PADI4 Polyclonal Antibody |
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A-2706 | EpiGentek |
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SMARCA5 Polyclonal Antibody |
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A-2707 | EpiGentek |
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IDH1 Polyclonal Antibody |
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A-2708 | EpiGentek |
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CHD4 Polyclonal Antibody |
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A-2709 | EpiGentek |
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CHAF1A Polyclonal Antibody |
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A-2710 | EpiGentek |
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BRD7 Polyclonal Antibody |
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A-2712 | EpiGentek |
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UHRF1 Polyclonal Antibody |
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A-2714 | EpiGentek |
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UHRF2 Polyclonal Antibody |
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A-2715 | EpiGentek |
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SMARCE1 Polyclonal Antibody |
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A-2716 | EpiGentek |
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ACTL6A Polyclonal Antibody |
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A-2717 | EpiGentek |
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MPG Polyclonal Antibody |
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A-2718 | EpiGentek |
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HIF1AN Polyclonal Antibody |
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A-2719 | EpiGentek |
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RNF2 Polyclonal Antibody |
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A-2720 | EpiGentek |
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PCGF6 Polyclonal Antibody |
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A-2721 | EpiGentek |
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SMARCB1 Polyclonal Antibody |
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A-2722 | EpiGentek |
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ALKBH3 Polyclonal Antibody |
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A-2723 | EpiGentek |
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BBOX1 Polyclonal Antibody |
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A-2724 | EpiGentek |
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BTAF1 Polyclonal Antibody |
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A-2725 | EpiGentek |
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CXXC1 Polyclonal Antibody |
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A-2726 | EpiGentek |
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KDM4B Polyclonal Antibody |
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A-2727 | EpiGentek |
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HELLS Polyclonal Antibody |
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A-2728 | EpiGentek |
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ING3 Polyclonal Antibody |
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A-2729 | EpiGentek |
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ING4 Polyclonal Antibody |
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A-2730 | EpiGentek |
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PHC1 Polyclonal Antibody |
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A-2731 | EpiGentek |
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SMARCAD1 Polyclonal Antibody |
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A-2732 | EpiGentek |
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USP16 Polyclonal Antibody |
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A-2733 | EpiGentek |
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SMARCC1 Polyclonal Antibody |
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A-2734 | EpiGentek |
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ERCC6L Polyclonal Antibody |
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A-2735 | EpiGentek |
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SMYD5 Polyclonal Antibody |
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A-2736 | EpiGentek |
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ATF2 Polyclonal Antibody |
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A-2738 | EpiGentek |
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BMI1 Polyclonal Antibody |
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A-2739 | EpiGentek |
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CTBP2 Polyclonal Antibody |
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A-2740 | EpiGentek |
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CTCF Polyclonal Antibody |
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A-2741 | EpiGentek |
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MTA2 Polyclonal Antibody |
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A-2742 | EpiGentek |
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PMS1 Polyclonal Antibody |
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A-2744 | EpiGentek |
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SFN Polyclonal Antibody |
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A-2745 | EpiGentek |
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SMARCA4 Polyclonal Antibody |
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A-2746 | EpiGentek |
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SMARCAL1 Polyclonal Antibody |
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A-2747 | EpiGentek |
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The components discovered to be considerably related to seropositivity towards anti-Fasciola IgG antibody had been the age group of 18 years outdated and above with calculated odds ratio of three.2 instances (p=0.032) and the period of farming actions of greater than 5 years with calculated odds ratio of two.6 instances (p=0.036). In conclusion, Fasciola an infection is prevalent amongst cattle farm staff and dwellers in Kelantan.