Is the level of HLA eplet mismatch a risk factor for graft loss among kidney transplant recipients who have already formed de novo donor specific antibody?

Is the level of HLA eplet mismatch a risk factor for graft loss among kidney transplant recipients who have already formed de novo donor specific antibody?

Is the level of HLA eplet mismatch a risk factor for graft loss among kidney transplant recipients who have already formed de novo donor specific antibody?

Eplet mismatches are related to de novo DSA (dnDSA) and antibody mediated rejection (ABMR) among the many common kidney transplant inhabitants. Nevertheless, it’s unclear whether or not the extent of eplet mismatch can be utilized for threat stratification amongst sufferers with dnDSA. We carried out a retrospective observational research of kidney transplant recipients with dnDSA (n = 44) transplanted between 10/2007 and 5/2014 to guage eplet mismatch as a threat issue for ABMR and allograft loss amongst dnDSA sufferers.

Excessive decision typing was inferred from by imputation primarily based on ethnicity and NMDP haplotypes, and the eplet mismatch was calculated utilizing the Epvix algorithm. Biopsies (N = 151) from 95.3%(42/44) of sufferers had been reviewed. The imply (SD) eplet mismatch was 69.8(22.8). The ABMR incidence was 71.4% (30/42) and 5 yr loss of life censored allograft survival was 67.4% throughout the imply (SD) follow-up of 5.3 (3.1) years. ABMR and death-censored allograft survival weren’t correlated with eplet mismatch amongst dnDSA sufferers.

Nevertheless, treatment adherence and dnDSA MFI < 3000 had been related to decreased ABMR incidence. Amongst sufferers with each of those favorable traits, solely 35.7% (15/42) developed ABMR. In conclusion, the extent of eplet mismatch doesn’t correlate with ABMR or allograft loss amongst excessive threat kidney transplant sufferers with dnDSA.

Excessive-throughput screening and validation of antibodies in opposition to synaptic proteins to discover opioid signaling dynamics

Antibodies symbolize highly effective instruments to look at sign transduction pathways. Right here, we current a technique integrating a number of state-of-the-art strategies to supply, validate, and make the most of antibodies. Specializing in understudied synaptic proteins, we generated 137 recombinant antibodies. We used yeast show antibody libraries from the B cells of immunized rabbits, adopted by FACS sorting beneath stringent circumstances to establish excessive affinity antibodies. The antibodies had been validated by high-throughput useful screening, and genome enhancing. Subsequent, we explored the temporal dynamics of signaling in single cells.
A subset of antibodies concentrating on opioid receptors had been used to look at the impact of therapy with opiates which have performed central roles within the worsening of the ‘opioid epidemic.’ We present that morphine and fentanyl exhibit differential temporal dynamics of receptor phosphorylation. In abstract, high-throughput approaches can result in the identification of antibody-based instruments required for an in-depth understanding of the temporal dynamics of opioid signaling.

SARS-CoV-2 B.1.1.7 sensitivity to mRNA vaccine-elicited, convalescent and monoclonal antibodies

Extreme Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) transmission is uncontrolled in lots of components of the world, compounded in some areas by increased transmission potential of the B1.1.7 variant now seen in 50 nations. It’s unclear whether or not responses to SARS-CoV-2 vaccines primarily based on the prototypic pressure will likely be impacted by mutations present in B.1.1.7. Right here we assessed immune responses following vaccination with mRNA-based vaccine BNT162b2. We measured neutralising antibody responses following a single immunization utilizing pseudoviruses expressing the wild-type Spike protein or the Eight amino acid mutations discovered within the B.1.1.7 spike protein.
The vaccine sera exhibited a broad vary of neutralising titres in opposition to the wild-type pseudoviruses that had been modestly decreased in opposition to B.1.1.7 variant. This discount was additionally evident in sera from some convalescent sufferers. Decreased B.1.1.7 neutralisation was additionally noticed with monoclonal antibodies concentrating on the N-terminal area (9 out of 10), the Receptor Binding Motif (RBM) (5 out of 31), however not in neutralising mAbs binding outdoors the RBM. Introduction of the E484Ok mutation in a B.1.1.7 background to replicate newly rising viruses within the UK led to a extra substantial lack of neutralising exercise by vaccine-elicited antibodies and mAbs (19 out of 31) over that conferred by the B.1.1.7 mutations alone. E484Ok emergence on a B.1.1.7 background represents a risk to the vaccine BNT162b.
Is the level of HLA eplet mismatch a risk factor for graft loss among kidney transplant recipients who have already formed de novo donor specific antibody?

Decreased neutralization of SARS-CoV-2 international variants by therapeutic anti-spike protein monoclonal antibodies

Monoclonal antibodies in opposition to the SARS-CoV-2 spike protein, notably, these developed by Regeneron Prescribed drugs and Eli Lilly and Firm have confirmed to supply safety in opposition to extreme COVID-19. The emergence of SARS-CoV-2 variants with closely mutated spike proteins raises the priority that the remedy might develop into much less efficient if any of the mutations disrupt epitopes engaged by the antibodies.
On this research, we examined monoclonal antibodies REGN10933 and REGN10987 which are utilized in mixture, for his or her capability to neutralize SARS-CoV-2 variants B.1.1.7, B.1.351, mink cluster 5 and COH.20G/677H. We report that REGN10987 maintains most of its neutralization exercise in opposition to viruses with B.1.1.7, B.1.351 and mink cluster 5 spike proteins however that REGN10933 has misplaced exercise in opposition to B.1.351 and mink cluster 5.
The failure of REGN10933 to neutralize B.1.351 is attributable to the Ok417N and E484Ok mutations within the receptor binding area; the failure to neutralize the mink cluster 5 spike protein is attributable to the Y453F mutation. The REGN10933 and REGN10987 mixture was 9.1-fold much less potent on B.1.351 and 16.2-fold much less potent on mink cluster 5, elevating considerations of decreased efficacy within the therapy of sufferers contaminated with variant viruses.

Mouse Placenta Growth Factor (PLGF) ELISA Kit
RD-PLGF-Mu-96Tests 96 Tests
EUR 400

Mouse Placenta Growth Factor (PLGF) ELISA Kit
RDR-PLGF-Mu-48Tests 48 Tests
EUR 307

Mouse Placenta Growth Factor (PLGF) ELISA Kit
RDR-PLGF-Mu-96Tests 96 Tests
EUR 418

anti-PLGF
YF-PA13759 50 ug
EUR 363
Description: Mouse polyclonal to PLGF

anti-PLGF
YF-PA13760 100 ug
EUR 403
Description: Rabbit polyclonal to PLGF

Bovine Placenta Growth Factor (PLGF) ELISA Kit
DLR-PLGF-b-48T 48T
EUR 493
  • Should the Bovine Placenta Growth Factor (PLGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Placenta Growth Factor (PLGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Placenta Growth Factor (PLGF) ELISA Kit
DLR-PLGF-b-96T 96T
EUR 641
  • Should the Bovine Placenta Growth Factor (PLGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Placenta Growth Factor (PLGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Placenta Growth Factor (PLGF) ELISA Kit
DLR-PLGF-Hu-48T 48T
EUR 337
  • Should the Human Placenta Growth Factor (PLGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Placenta Growth Factor (PLGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Placenta Growth Factor (PLGF) ELISA Kit
DLR-PLGF-Hu-96T 96T
EUR 426
  • Should the Human Placenta Growth Factor (PLGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Placenta Growth Factor (PLGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Placenta Growth Factor (PLGF) ELISA Kit
DLR-PLGF-Ra-48T 48T
EUR 454
  • Should the Rat Placenta Growth Factor (PLGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Placenta Growth Factor (PLGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Placenta Growth Factor (PLGF) ELISA Kit
DLR-PLGF-Ra-96T 96T
EUR 587
  • Should the Rat Placenta Growth Factor (PLGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Placenta Growth Factor (PLGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Placenta Growth Factor (PLGF) ELISA Kit
RD-PLGF-b-48Tests 48 Tests
EUR 494

Bovine Placenta Growth Factor (PLGF) ELISA Kit
RD-PLGF-b-96Tests 96 Tests
EUR 684

Human Placenta Growth Factor (PLGF) ELISA Kit
RD-PLGF-Hu-48Tests 48 Tests
EUR 318

Human Placenta Growth Factor (PLGF) ELISA Kit
RD-PLGF-Hu-96Tests 96 Tests
EUR 432

Rat Placenta Growth Factor (PLGF) ELISA Kit
RD-PLGF-Ra-48Tests 48 Tests
EUR 450

Rat Placenta Growth Factor (PLGF) ELISA Kit
RD-PLGF-Ra-96Tests 96 Tests
EUR 622

Bovine Placenta Growth Factor (PLGF) ELISA Kit
RDR-PLGF-b-48Tests 48 Tests
EUR 516

Bovine Placenta Growth Factor (PLGF) ELISA Kit
RDR-PLGF-b-96Tests 96 Tests
EUR 716

Human Placenta Growth Factor (PLGF) ELISA Kit
RDR-PLGF-Hu-48Tests 48 Tests
EUR 331

Human Placenta Growth Factor (PLGF) ELISA Kit
RDR-PLGF-Hu-96Tests 96 Tests
EUR 451

Rat Placenta Growth Factor (PLGF) ELISA Kit
RDR-PLGF-Ra-48Tests 48 Tests
EUR 470

Rat Placenta Growth Factor (PLGF) ELISA Kit
RDR-PLGF-Ra-96Tests 96 Tests
EUR 651

Anti-PLGF antibody
STJ72011 100 µg
EUR 359

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349

Anti-PLGF-2 Antibody
A1303-100
EUR 338

Anti-PLGF-2 Antibody
A1303-30T
EUR 146

Anti-PLGF/PGF Antibody
PA1066 100ug/vial
EUR 294

Polyclonal Goat Anti-PLGF Antibody
AMM05090G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-PLGF . This antibody is tested and proven to work in the following applications:

Mouse PLGF ELISA Kit
EMP0025 96Tests
EUR 521

PLGF Antibody
49602-100ul 100ul
EUR 333

PLGF Antibody
49602-50ul 50ul
EUR 239

PLGF protein
30R-2693 10 ug
EUR 325
Description: Purified recombinant Mouse PLGF protein

PlGF Antibody
5743-100
EUR 338

PlGF Antibody
5743-30T
EUR 146

PlGF antibody
70R-12427 100 ug
EUR 436
Description: Rabbit polyclonal PlGF antibody

Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 280

Recombinant Mouse PLGF/PGF Protein
RP01080 10 μg
EUR 234

Polyclonal PlGF Antibody
AMR09382G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PlGF . This antibody is tested and proven to work in the following applications:

PLGF Conjugated Antibody
C49602 100ul
EUR 397

PLGF(PLGF93) Antibody
BNC610093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF660R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC610093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF660R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC610094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF660R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC610094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF660R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC680093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF568 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC680093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF568 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC680094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF568 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC680094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF568 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC430093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF543 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC430093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF543 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC430094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF543 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC430094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF543 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC550093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF555 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC550093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF555 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC550094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF555 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC550094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF555 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC040093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF405S conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC040093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF405S conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC040094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF405S conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC040094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF405S conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC400093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF640R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC400093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF640R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC400094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF640R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC400094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF640R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC050093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF405M conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC050093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF405M conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC050094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF405M conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC050094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF405M conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC470093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF647 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC470093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF647 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC470094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF647 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC470094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF647 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNUM0093-50 50uL
EUR 395
Description: Primary antibody against PLGF(PLGF93), 1mg/mL

PLGF(PLGF94) Antibody
BNUM0094-50 50uL
EUR 395
Description: Primary antibody against PLGF(PLGF94), 1mg/mL

PLGF(PLGF93) Antibody
BNC700093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF770 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC700093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF770 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC700094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF770 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC700094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF770 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC940093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF594 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC940093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF594 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC940094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF594 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC940094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF594 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNCA0093-250 250uL
EUR 383
Description: Primary antibody against PLGF(PLGF93), APC conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNCA0094-250 250uL
EUR 383
Description: Primary antibody against PLGF(PLGF94), APC conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNCH0093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNCH0093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNCH0094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNCH0094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC800093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF680 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC800093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF680 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC800094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF680 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC800094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF680 conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC810093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF680R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC810093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF680R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC810094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF680R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC810094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF680R conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNCP0093-250 250uL
EUR 383
Description: Primary antibody against PLGF(PLGF93), PerCP conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNCP0094-250 250uL
EUR 383
Description: Primary antibody against PLGF(PLGF94), PerCP conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNCR0093-250 250uL
EUR 383
Description: Primary antibody against PLGF(PLGF93), RPE conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNCR0094-250 250uL
EUR 383
Description: Primary antibody against PLGF(PLGF94), RPE conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNCAP0093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNCAP0093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNCAP0094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNCAP0094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNCB0093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), Biotin conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNCB0093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), Biotin conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNCB0094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), Biotin conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNCB0094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), Biotin conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC880093-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF93), CF488A conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNC880093-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF93), CF488A conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC880094-100 100uL
EUR 199
Description: Primary antibody against PLGF(PLGF94), CF488A conjugate, Concentration: 0.1mg/mL

PLGF(PLGF94) Antibody
BNC880094-500 500uL
EUR 544
Description: Primary antibody against PLGF(PLGF94), CF488A conjugate, Concentration: 0.1mg/mL

PLGF(PLGF93) Antibody
BNUB0093-100 100uL
EUR 209
Description: Primary antibody against PLGF(PLGF93), Concentration: 0.2mg/mL

PLGF(PLGF93) Antibody
BNUB0093-500 500uL
EUR 458
Description: Primary antibody against PLGF(PLGF93), Concentration: 0.2mg/mL

PLGF(PLGF94) Antibody
BNUB0094-100 100uL
EUR 209
Description: Primary antibody against PLGF(PLGF94), Concentration: 0.2mg/mL

PLGF(PLGF94) Antibody
BNUB0094-500 500uL
EUR 458
Description: Primary antibody against PLGF(PLGF94), Concentration: 0.2mg/mL

PLGF Rabbit pAb
A1727-100ul 100 ul
EUR 308

PLGF Rabbit pAb
A1727-200ul 200 ul
EUR 459

PLGF Rabbit pAb
A1727-20ul 20 ul
EUR 183

PLGF Rabbit pAb
A1727-50ul 50 ul
EUR 223

PLGF Rabbit pAb
A16257-100ul 100 ul
EUR 308

PLGF Rabbit pAb
A16257-200ul 200 ul
EUR 459

PLGF Rabbit pAb
A16257-20ul 20 ul
EUR 183

PLGF Rabbit pAb
A16257-50ul 50 ul
EUR 223

PLGF, murine recombinant
4743-10
EUR 245

PLGF, murine recombinant
4743-1000
EUR 5433

PLGF, murine recombinant
4743-50
EUR 756

PlGF-1 Antibody
5739-100
EUR 338

PlGF-1 Antibody
5739-30T
EUR 146

Mouse PLGF-2 PicoKine ELISA Kit
EK0863 96 wells
EUR 425
Description: For Quantitative Detection of mouse PLGF-2 in cell culture supernates, serum and plasma (heparin, EDTA).

Mouse Placenta Growth Factor (PLGF) Protein
20-abx068553
  • EUR 982.00
  • EUR 342.00
  • EUR 3195.00
  • EUR 1177.00
  • EUR 676.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

Polyclonal PlGF-1 Antibody
AMR09383G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PlGF-1 . This antibody is tested and proven to work in the following applications:

Human PLGF ELISA Kit
EHP0025 96Tests
EUR 521

Goat PLGF ELISA Kit
EGTP0025 96Tests
EUR 521

Bovine PLGF ELISA Kit
EBP0025 96Tests
EUR 521

Chicken PLGF ELISA Kit
ECKP0025 96Tests
EUR 521

Canine PLGF ELISA Kit
ECP0025 96Tests
EUR 521

Anserini PLGF ELISA Kit
EAP0025 96Tests
EUR 521

PLGF ELISA KIT|Human
EF000015 96 Tests
EUR 689

Porcine PLGF ELISA Kit
EPP0025 96Tests
EUR 521

Rat PLGF ELISA Kit
ERP0025 96Tests
EUR 521

Rabbit PLGF ELISA Kit
ERTP0025 96Tests
EUR 521

Sheep PLGF ELISA Kit
ESP0025 96Tests
EUR 521

Monkey PLGF ELISA Kit
EMKP0025 96Tests
EUR 521

PLGF-1, human recombinant
4739-100
EUR 697

PLGF-1, human recombinant
4739-1000
EUR 3258

PLGF-1, human recombinant
4739-25
EUR 256

PLGF-2, human recombinant
4741-10
EUR 256

PLGF-2, human recombinant
4741-1000
EUR 3421

PLGF-2, human recombinant
4741-50
EUR 729

Human PLGF ELISA kit
LF-EK50333 1×96T
EUR 648

PLGF-3 Human Recombinant
P1563-10 10 µg
EUR 176

PLGF-3 Human Recombinant
P1563-50 50 µg
EUR 551

Mouse Placenta growth factor, PlGF ELISA Kit
ELA-E0114m 96 Tests
EUR 865

Mouse placenta growth factor(PLGF)ELISA Kit 　
GA-E0088MS-48T 48T
EUR 336

Mouse placenta growth factor(PLGF)ELISA Kit 　
GA-E0088MS-96T 96T
EUR 534

Mouse placenta growth factor, PLGF ELISA kit
CSB-E07401m-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Mouse placenta growth factor, PLGF in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse placenta growth factor, PLGF ELISA kit
1-CSB-E07401m
  • EUR 723.00
  • EUR 4883.00
  • EUR 2591.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativecompetitive ELISA kit for measuring Mouse placenta growth factor, PLGF in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse placenta growth factor,PLGF ELISA kit
CN-02373M1 96T
EUR 471

Mouse placenta growth factor,PLGF ELISA kit
CN-02373M2 48T
EUR 322

Placenta Growth Factor (PLGF) Polyclonal Antibody (Mouse)
4-PAA114Mu01
  • EUR 190.00
  • EUR 1612.00
  • EUR 421.00
  • EUR 228.00
  • EUR 189.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PLGF (Val19~Pro158)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Placenta Growth Factor (PLGF)

Mouse Placenta Growth Factor (PLGF) ELISA Kit
SEA114Mu-10x96wellstestplate 10x96-wells test plate
EUR 3409.41
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Placenta Growth Factor (PLGF) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Placenta Growth Factor (PLGF) ELISA Kit
SEA114Mu-1x48wellstestplate 1x48-wells test plate
EUR 368.42
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Placenta Growth Factor (PLGF) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Placenta Growth Factor (PLGF) ELISA Kit
SEA114Mu-1x96wellstestplate 1x96-wells test plate
EUR 483.46
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Placenta Growth Factor (PLGF) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Placenta Growth Factor (PLGF) ELISA Kit
SEA114Mu-5x96wellstestplate 5x96-wells test plate
EUR 1875.57
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Placenta Growth Factor (PLGF) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Placenta Growth Factor (PLGF) ELISA Kit
4-SEA114Mu
  • EUR 3460.00
  • EUR 1826.00
  • EUR 484.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Placenta Growth Factor elisa. Alternative names of the recognized antigen: PlGF2
  • PGF
  • PGFL
  • Placental Growth Factor-Like
  • Vascular Endothelial Growth Factor-Related Protein
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Placenta Growth Factor (PLGF) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse placenta growth factor(PLGF)ELISA Kit
QY-E21188 96T
EUR 361

ELISA kit for Human PLGF
EK5213 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human PLGF in samples from serum, plasma, tissue homogenates and other biological fluids.

Human PLGF PicoKine ELISA Kit
EK0490 96 wells
EUR 436
Description: For quantitative detection of human PLGF in cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.

Guinea Pig PLGF ELISA Kit
EGP0025 96Tests
EUR 521

Placenta Growth Factor (PLGF) Antibody
20-abx142140
  • EUR 370.00
  • EUR 606.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

Placenta Growth Factor (PLGF) Antibody
20-abx102283
  • EUR 300.00
  • EUR 133.00
  • EUR 801.00
  • EUR 425.00
  • EUR 258.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Placenta Growth Factor (PLGF) Antibody
20-abx102284
  • EUR 314.00
  • EUR 133.00
  • EUR 857.00
  • EUR 439.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Placenta Growth Factor (PLGF) Antibody
20-abx102285
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Placenta Growth Factor (PLGF) Antibody
20-abx174070
  • EUR 704.00
  • EUR 356.00
  • 1 mg
  • 200 ug
  • Please enquire.

Placenta Growth Factor (PLGF) Antibody
20-abx174071
  • EUR 843.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Placenta Growth Factor (PLGF) Antibody
20-abx178023
  • EUR 1135.00
  • EUR 551.00
  • 1 mg
  • 200 ug
  • Please enquire.

Placenta Growth Factor (PLGF) Antibody
20-abx178024
  • EUR 1288.00
  • EUR 620.00
  • 1 mg
  • 200 ug
  • Please enquire.

Placenta Growth Factor (PLGF) Antibody
abx433146-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

PLGF-2, human recombinant protein
P1056-.01 10 µg
EUR 300
Description: Placenta Growth Factor-2 (PLGF-2) is an angiogenic factor produced in umbilical vein endothelial cells and placenta. PLGF-2 plays important roles in angiogenesis and endothelial cell growth.

PLGF-2, human recombinant protein
P1056-.05 50 µg
EUR 877
Description: Placenta Growth Factor-2 (PLGF-2) is an angiogenic factor produced in umbilical vein endothelial cells and placenta. PLGF-2 plays important roles in angiogenesis and endothelial cell growth.

PLGF-2, human recombinant protein
P1056-1 1 mg
EUR 4156
Description: Placenta Growth Factor-2 (PLGF-2) is an angiogenic factor produced in umbilical vein endothelial cells and placenta. PLGF-2 plays important roles in angiogenesis and endothelial cell growth.

Human PLGF ELISA kit (4X96T)
LF-EK50334 4×96T
EUR 2201

Recombinant Placenta Growth Factor (PLGF)
4-RPA114Hu01
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P49763
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 17.8kDa
  • Isoelectric Point: 6.8
Description: Recombinant Human Placenta Growth Factor expressed in: E.coli

Recombinant Placenta Growth Factor (PLGF)
4-RPA114Mu01
  • EUR 709.28
  • EUR 293.00
  • EUR 2384.80
  • EUR 861.60
  • EUR 1623.20
  • EUR 538.00
  • EUR 5812.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P49764
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 47.9kDa
  • Isoelectric Point: 6.3
Description: Recombinant Mouse Placenta Growth Factor expressed in: E.coli

Recombinant Placenta Growth Factor (PLGF)
4-RPA114Ra01
  • EUR 535.46
  • EUR 246.00
  • EUR 1732.96
  • EUR 644.32
  • EUR 1188.64
  • EUR 421.00
  • EUR 4182.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q63434
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 19.0kDa
  • Isoelectric Point: 7.7
Description: Recombinant Rat Placenta Growth Factor expressed in: E.coli

Recombinant Human PlGF-3 Protein
PROTP49763-6 25ug
EUR 317
Description: PlGF-3 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-3 is expressed exclusively in the placenta. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration. PlGF-3 lacks heparin binding affinity. Recombinant human PlGF-3 is a 45.7 kDa disulfide-linked homodimeric protein of two 203 amino acid polypeptide chains.

Recombinant Human PlGF-1 Protein
PROTP49763-7 25ug
EUR 317
Description: PlGF-1 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-1 is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration. Recombinant human PlGF-1 is a 29.7 kDa disulfide-linked homodimeric protein of two 132 amino acid polypeptide chains.

Recombinant Human PlGF-2 Protein
PROTP49763-8 25ug
EUR 317
Description: PLGF-2 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PLGF-2 is expressed in umbilical vein endothelial cells and placenta. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration. PlGF-2 also signals through Neuropilin (NP-1) and can bind with high affinity to heparin. Recombinant human PLGF-2 is a 34.0 kDa disulfide-linked homodimeric protein of two 150 amino acid polypeptide chains.

PLGF ELISA Kit (Human) (OKBB00242)
OKBB00242 96 Tests
EUR 518
Description: Description of target: The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 1 pg/mL

PLGF ELISA Kit (Cattle) (OKCD04426)
OKCD04426 96 Wells
EUR 936
Description: Description of target: ;Species reactivity: Cattle;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 5.9 pg/mL

ELISA kit for Mouse PLGF (Placenta Growth Factor)
ELK1242 1 plate of 96 wells
EUR 372
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placenta Growth Factor (PLGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plac
  • Show more
Description: A sandwich ELISA kit for detection of Placenta Growth Factor from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Placenta Growth Factor (PLGF) Polyclonal Antibody (Mouse), APC
4-PAA114Mu01-APC
  • EUR 260.00
  • EUR 2051.00
  • EUR 606.00
  • EUR 317.00
  • EUR 182.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PLGF (Val19~Pro158)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Placenta Growth Factor (PLGF). This antibody is labeled with APC.

Placenta Growth Factor (PLGF) Polyclonal Antibody (Mouse), Biotinylated
4-PAA114Mu01-Biotin
  • EUR 249.00
  • EUR 1562.00
  • EUR 503.00
  • EUR 291.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PLGF (Val19~Pro158)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Placenta Growth Factor (PLGF). This antibody is labeled with Biotin.

Placenta Growth Factor (PLGF) Polyclonal Antibody (Mouse), Cy3
4-PAA114Mu01-Cy3
  • EUR 305.00
  • EUR 2693.00
  • EUR 767.00
  • EUR 381.00
  • EUR 201.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PLGF (Val19~Pro158)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Placenta Growth Factor (PLGF). This antibody is labeled with Cy3.

Placenta Growth Factor (PLGF) Polyclonal Antibody (Mouse), FITC
4-PAA114Mu01-FITC
  • EUR 228.00
  • EUR 1660.00
  • EUR 505.00
  • EUR 274.00
  • EUR 166.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PLGF (Val19~Pro158)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Placenta Growth Factor (PLGF). This antibody is labeled with FITC.

Placenta Growth Factor (PLGF) Polyclonal Antibody (Mouse), HRP
4-PAA114Mu01-HRP
  • EUR 242.00
  • EUR 1794.00
  • EUR 542.00
  • EUR 291.00
  • EUR 174.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PLGF (Val19~Pro158)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Placenta Growth Factor (PLGF). This antibody is labeled with HRP.

Placenta Growth Factor (PLGF) Polyclonal Antibody (Mouse), PE
4-PAA114Mu01-PE
  • EUR 228.00
  • EUR 1660.00
  • EUR 505.00
  • EUR 274.00
  • EUR 166.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PLGF (Val19~Pro158)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Placenta Growth Factor (PLGF). This antibody is labeled with PE.

ELISA kit for Mouse Placenta growth factor (PLGF)
KTE70764-48T 48T
EUR 381
  • Placental growth factor (PLGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PLGF during pregnancy is the placental
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Placenta growth factor (PLGF) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
The outcomes recommend that there’s a must develop further monoclonal antibodies that aren’t affected by the present spike protein mutations.
Antibodies where obtained from Orla Proteins Ltd. London.
Tags :  , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,

Leave a Reply

Your email address will not be published. Required fields are marked *