Integrin Research The Martin Humphries Lab

Identification of critical chemical modifications and paratope mapping by size exclusion chromatography of stressed antibody-target complexes

Therapeutic proteins together with antibodies and Fc-fusion proteins bear numerous chemical modifications throughout cell tradition, purification, storage and in human circulation. They’re additionally uncovered to harsh situations throughout stress research, together with elevated temperature, extremes of pH, pressured oxidation, physiological pH, UV mild to evaluate the potential degradation pathways and suitability of strategies for detecting them. A few of these modifications are positioned on residues in binding areas, resulting in lack of binding and efficiency and categorised as vital high quality attributes.

At present, criticality of modifications is assessed by a laborious means of amassing antibody fractions from the mushy chromatography strategies ion change and hydrophobic interplay chromatography and characterizing the fractions one-by-one for efficiency and chemical modifications. Right here, we describe a technique for large-scale, parallel identification of all vital chemical modifications in a single experiment.

In step one, the antibody is harassed by one or a number of stress strategies. It’s then blended with goal protein and separated by size-exclusion chromatography (SEC) on sure antibody-target complicated and unbound antibody. Peptide mapping of fractions and statistical evaluation are carried out to establish modifications on amino acid residues that have an effect on binding.

To establish the modifications resulting in slight decreases in binding, aggressive SEC of antibody and antigen mixtures was developed and described in a companion research by Shi et al, the place goal protein is supplied at decrease stage, beneath the stoichiometry. The newly described methodology was efficiently correlated to crystallography for assessing criticality of chemical modifications and paratope mapping. It’s extra delicate to low-level modifications, higher streamlined and platform prepared.

Identification of vital chemical modifications by dimension exclusion chromatography of harassed antibody-target complexes with aggressive binding

Chemical modifications (attributes) within the binding areas of harassed therapeutic proteins might have an effect on binding to focus on and efficacy of therapeutic proteins. The tactic introduced right here describes the criticality evaluation of therapeutic antibody modifications by size-exclusion chromatography (SEC) of aggressive binding between a harassed antibody and its goal, human epidermal progress issue receptor-2 (HER2), adopted by SEC fractionation and peptide mapping characterization of sure and unbound antibodies.

When harassed antibody and its goal had been blended at a stoichiometric molar ratio of 1:2, solely antibody-receptor complicated eluted from SEC, indicating that binding was not decreased to interrupt the complicated. When a smaller quantity of the receptor was supplied (1:1), the antibody species with modifications decreasing binding eluted as unbound from SEC, whereas the antibody-receptor complicated eluted because the sure fraction. Peptide mapping revealed ratios of modifications between unbound and sure fractions.

Statistical evaluation after triplicate measurements (n = 3) indicated that heavy chain (HC) D102 isomerization and light-weight chain (LC) N30 deamidation had been four-fold greater in unbound fraction with excessive statistical significance. Though HC N55 deamidation and M107 oxidation had been additionally ample, they weren’t statistically completely different between unbound and sure.

Our findings agree with beforehand printed efficiency measurements of collected CEX fractions and the crystal construction of antibody and HER2. General, aggressive SEC of harassed antibody-receptor combination adopted by peptide mapping is a great tool in revealing vital residues and modifications concerned within the antibody-target binding, even when they elute as a posh from SEC when blended at 1:2 stoichiometric ratio.

Fast Dedication of Antibody-Antigen Affinity by Mass Photometry

Measurements of the specificity and affinity of antigen-antibody interactions are critically necessary for medical and analysis purposes. On this protocol, we describe the implementation of a brand new single-molecule method, mass photometry (MP), for this goal. MP is a label- and immobilization-free method that detects and quantifies molecular lots and populations of antibodies and antigen-antibody complexes on a single-molecule stage. MP analyzes the antigen-antibody pattern inside minutes, permitting for the exact dedication of the binding affinity and concurrently offering data on the stoichiometry and the oligomeric state of the proteins.

It is a easy and simple method that requires solely picomole portions of protein and no costly consumables. The identical process can be utilized to review protein-protein binding for proteins with a molecular mass bigger than 50 kDa. For multivalent protein interactions, the affinities of a number of binding websites might be obtained in a single measurement.

Nevertheless, the single-molecule mode of measurement and the shortage of labeling imposes some experimental limitations. This methodology offers the most effective outcomes when utilized to measurements of sub-micromolar interplay affinities, antigens with a molecular mass of 20 kDa or bigger, and comparatively pure protein samples. We additionally describe the process for performing the required becoming and calculation steps utilizing primary information evaluation software program.

Detection and distribution of anti-leptospiral antibody amongst canine and their handlers

The incidence of leptospirosis appears to be on the rise and could possibly be an alarming oblique indication of a world re-emergence. It’s a potential public well being menace when canine are alleged to be concerned within the transmission of leptospirosis by means of potential subclinical harbouring of Leptospira spp. and subsequent shedding into the atmosphere. This research aimed to detect anti-leptospiral antibodies amongst canine and their handlers utilizing the microscopic agglutination take a look at (MAT).

Blood samples from 266 apparently wholesome canine and 194 canine handlers had been collected at 4 working canine organisations and 4 canine shelters. Serum samples had been examined utilizing MAT in opposition to 20 leptospiral serovars with a cut-off titre >=1:100 (canine) and >=1:50 (canine handlers). Seventy canine (70/266; 26.3%) had been seropositive primarily in opposition to serovars Icterohaemorrhagiae, Ballum, Bataviae and Javanica (titres ranged: 1:100-1:800).

Sixty-seven canine handlers (67/194; 34.5%) had been seropositive primarily in opposition to serovars Grippotyphosa, Icterohaemorrhagiae and Malaysia (titres ranged: 1:50-1:200). Canines had been seropositive as a result of publicity, vaccination or lively an infection. Seropositive canine handlers may point out publicity or lively an infection.

D-Dimer Antibody
abx020765-1mg	Abbexa	1 mg	EUR 739

D-Dimer Antibody
abx020767-1mg	Abbexa	1 mg	EUR 1233

D-Dimer Antibody
abx020769-1mg	Abbexa	1 mg	EUR 739

D-Dimer Antibody
abx020770-1mg	Abbexa	1 mg	EUR 739

D-Dimer Antibody
abx020771-1mg	Abbexa	1 mg	EUR 739

D-Dimer Antibody
abx023583-1mg	Abbexa	1 mg	EUR 739

D-Dimer Antibody
abx023584-1mg	Abbexa	1 mg	EUR 739

D-Dimer antibody
10R-D100a	Fitzgerald	1 mg	EUR 535
Description: Mouse monoclonal D-Dimer antibody

D-Dimer antibody
10R-D100C	Fitzgerald	1 mg	EUR 535
Description: Mouse monoclonal D-Dimer antibody

D-Dimer antibody
10-1155	Fitzgerald	250 ug	EUR 356
Description: Mouse monoclonal D-Dimer antibody

D-dimer antibody
10-1752	Fitzgerald	200 ul	EUR 543
Description: Mouse monoclonal D-dimer antibody

D-dimer antibody
10-1753	Fitzgerald	200 ul	EUR 543
Description: Mouse monoclonal D-dimer antibody

D-dimer antibody
10-2259	Fitzgerald	1 mg	EUR 349
Description: Mouse monoclonal D-dimer antibody

D-dimer antibody
10-7900	Fitzgerald	1 mg	EUR 535
Description: Mouse monoclonal D-dimer antibody

D-dimer antibody
10-D100A	Fitzgerald	1 mg	EUR 535
Description: Mouse monoclonal D-dimer antibody

D-Dimer antibody
10-D42A	Fitzgerald	1 mg	EUR 535
Description: Mouse monoclonal D-Dimer antibody

D-Dimer antibody
10-D42B	Fitzgerald	1 mg	EUR 535
Description: Mouse monoclonal D-Dimer antibody

Human D-Dimer Antibody
30191-05111	AssayPro	150 ug	EUR 261

Anti-D-Dimer antibody
STJ400061	St John's Laboratory	1 mg	EUR 446
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400062	St John's Laboratory	1 mg	EUR 446
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400063	St John's Laboratory	1 mg	EUR 446
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400064	St John's Laboratory	1 mg	EUR 446
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400065	St John's Laboratory	1 mg	EUR 446
Description: D-Dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-Dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-Dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400067	St John's Laboratory	1 mg	EUR 446
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

D-Dimer Antibody (Biotin)
abx020757-1mg	Abbexa	1 mg	EUR 1372

D-Dimer Antibody (Biotin)
20-abx272516	Abbexa	EUR 398.00 EUR 230.00 EUR 1080.00 EUR 537.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

D-Dimer monoclonal antibody
10R-11476	Fitzgerald	1 mg	EUR 462
Description: Mouse anti-human D-Dimer monoclonal antibody

D-dimer antibody (biotin)
61-1009	Fitzgerald	100 ul	EUR 813
Description: Mouse monoclonal D-dimer antibody (Biotin)

D-dimer Antigen
E64C00801	EnoGene	1mg	EUR 1675

D-dimer Antigen
E64C00802	EnoGene	100ug	EUR 343

D-Dimer Protein
abx061440-1mg	Abbexa	1 mg	EUR 1358

D-Dimer Protein
abx069809-1mg	Abbexa	1 mg	EUR 1372

D-Dimer protein
30-1099S	Fitzgerald	100 ug	EUR 244
Description: Purified native Human D-Dimer protein

D Dimer protein
30-1978	Fitzgerald	1 mg	EUR 912
Description: Human D Dimer protein

D-Dimer protein
30R-AD001	Fitzgerald	1 mg	EUR 1709
Description: Purified native Human D-Dimer protein

Anti-D-Dimer Detection Antibody
A2090-100	Biovision	100 µg	EUR 338

Anti-D-Dimer Detection Antibody
A2091-100	Biovision	100 µg	EUR 338

Human D-Dimer Protein
abx670031-02mg	Abbexa	0.2 mg	EUR 759

D-Dimer Assay Kit
abx098424-Hitachi7020R145ml1R215ml1	Abbexa	Hitachi 7020; R1:45ml×1 R2:15ml×1	EUR 2007

D-Dimer Assay Kit
abx098424-Hitachi7060R145ml1R215ml1	Abbexa	Hitachi 7060; R1:45ml×1 R2:15ml×1	EUR 2007

D-Dimer Assay Kit
abx098424-Hitachi7170R130ml1R210ml1	Abbexa	Hitachi 7170; R1:30ml×1 R2:10ml×1	EUR 1396

D-Dimer Assay Kit
abx098424-Hitachi7170R145ml1R215ml1	Abbexa	Hitachi 7170; R1:45ml×1 R2:15ml×1	EUR 2007

D-Dimer Assay Kit
abx098424-Toshiba40R145ml1R215ml1	Abbexa	Toshiba 40; R1:45ml×1 R2:15ml×1	EUR 2007

Human D-Dimer Protein
20-abx060114	Abbexa	EUR 258.00 EUR 565.00	100 ug 1 mg

Human D-Dimer Protein
abx060234-1mg	Abbexa	1 mg	EUR 565

Human D-Dimer Protein
abx060717-100ug	Abbexa	100 ug	EUR 1295

D-Dimer (Capture Ab)
abx019235-1mg	Abbexa	1 mg	EUR 537

D-Dimer (Capture Ab)
abx019236-1mg	Abbexa	1 mg	EUR 537

D-Dimer (Detection Ab)
abx019237-1mg	Abbexa	1 mg	EUR 537

D-Dimer, Human Plasma
P1434-100	Biovision		EUR 207

D-Dimer, Human Plasma
P1434-1000	Biovision		EUR 604

D-Dimer (D2D) Polyclonal Antibody (Human)
4-PAA506Hu01	Cloud-Clone	EUR 217.00 EUR 2048.00 EUR 520.00 EUR 268.00 EUR 201.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D)

Human D-Dimer Antibody (Biotin Conjugate)
30191-05121	AssayPro	150 ug	EUR 369

OVA Conjugated D-Dimer (D2D)
4-CPA506Hu21	Cloud-Clone	EUR 413.60 EUR 214.00 EUR 1276.00 EUR 492.00 EUR 884.00 EUR 340.00 EUR 3040.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human D-Dimer expressed in: Protein conjugation

Human D-dimer ELISA Kit
PRB-5048	Cell Biolabs	96 assays	EUR 572
Description: Cell Biolabs? Human D-dimer ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human D-dimer in plasma, serum or other biological fluid samples.  The kit has a detection sensitivity limit of 150 pg/mL human D-dimer.  Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

Human D-dimer ELISA Kit
PRB-5048-5	Cell Biolabs	5 x 96 assays	EUR 2283
Description: Cell Biolabs? Human D-dimer ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human D-dimer in plasma, serum or other biological fluid samples.  The kit has a detection sensitivity limit of 150 pg/mL human D-dimer.  Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

Human D-DIMER ELISA Kit
DEIADD2911	Creative Diagnostics	96T	EUR 627
Description: The CD Human D-DIMER ELISA Kit is an enzyme-linked immunosorbent assay for measuring Human DDIMER in serum, plasma, and cell culture media.

Rat D-Dimer ELISA Kit
abx575948-96tests	Abbexa	96 tests	EUR 786

Monkey D-Dimer ELISA Kit
abx353336-96tests	Abbexa	96 tests	EUR 825

Dog D-Dimer ELISA Kit
abx354632-96tests	Abbexa	96 tests	EUR 926

Chicken D-Dimer ELISA Kit
abx354774-96tests	Abbexa	96 tests	EUR 825

Mouse D-Dimer Protein (OVA)
20-abx655872	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug

Pig D-Dimer ELISA Kit
abx051362-96tests	Abbexa	96 tests	EUR 825

Human D-Dimer ELISA Kit
20-abx151266	Abbexa	EUR 6173.00 EUR 3291.00 EUR 770.00	10 × 96 tests 5 × 96 tests 96 tests

Horse D-Dimer ELISA Kit
20-abx150441	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Human D-Dimer CLIA Kit
abx196592-96tests	Abbexa	96 tests	EUR 825

Human D-Dimer ELISA Kit
abx253171-96tests	Abbexa	96 tests	EUR 754

Human D-Dimer Protein (OVA)
20-abx165525	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug

Pig D-Dimer ELISA Kit
20-abx154909	Abbexa	EUR 7645.00 EUR 4074.00 EUR 942.00	10 × 96 tests 5 × 96 tests 96 tests

Rabbit D-Dimer ELISA Kit
20-abx155054	Abbexa	EUR 7237.00 EUR 3855.00 EUR 895.00	10 × 96 tests 5 × 96 tests 96 tests

Rat D-Dimer ELISA Kit
20-abx155431	Abbexa	EUR 7237.00 EUR 3855.00 EUR 895.00	10 × 96 tests 5 × 96 tests 96 tests

Rat D-Dimer ELISA Kit
abx256862-96tests	Abbexa	96 tests	EUR 786

Rabbit D-Dimer ELISA Kit
abx257004-96tests	Abbexa	96 tests	EUR 786

Pig D-Dimer ELISA Kit
abx257608-96tests	Abbexa	96 tests	EUR 825

Mouse D-Dimer ELISA Kit
20-abx258705	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Rat D-Dimer ELISA Kit
20-abx258706	Abbexa	EUR 6642.00 EUR 3542.00 EUR 825.00	10 × 96 tests 5 × 96 tests 96 tests

Horse D-Dimer CLIA Kit
20-abx490143	Abbexa	EUR 8569.00 EUR 4560.00 EUR 1052.00	10 × 96 tests 5 × 96 tests 96 tests

Human D-Dimer CLIA Kit
20-abx490144	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Pig D-Dimer CLIA Kit
20-abx490145	Abbexa	EUR 8569.00 EUR 4560.00 EUR 1052.00	10 × 96 tests 5 × 96 tests 96 tests

Rat D-Dimer CLIA Kit
20-abx490146	Abbexa	EUR 8569.00 EUR 4560.00 EUR 1052.00	10 × 96 tests 5 × 96 tests 96 tests

Rabbit D-Dimer CLIA Kit
20-abx490147	Abbexa	EUR 8569.00 EUR 4560.00 EUR 1052.00	10 × 96 tests 5 × 96 tests 96 tests

Rat D-Dimer CLIA Kit
20-abx490570	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

Porcine D- Dimer ELISA Kit
ELA-E0506p	Lifescience Market	96 Tests	EUR 928

Rabbit D- Dimer ELISA Kit
ELA-E0506Rb	Lifescience Market	96 Tests	EUR 928

Rat D Dimer ELISA kit
E02D0224-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat D Dimer ELISA kit
E02D0224-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat D Dimer ELISA kit
E02D0224-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey D Dimer ELISA kit
E09D0224-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey D Dimer ELISA kit
E09D0224-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey D Dimer ELISA kit
E09D0224-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

D-Dimer protein (> 98% pure)
30-1021	Fitzgerald	100 ug	EUR 673
Description: Purified native Human D-Dimer protein

Human D Dimer ELISA kit
E01D0224-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human D Dimer ELISA kit
E01D0224-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human D Dimer ELISA kit
E01D0224-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit D Dimer ELISA kit
E04D0224-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit D Dimer ELISA kit
E04D0224-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit D Dimer ELISA kit
E04D0224-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse D Dimer ELISA kit
E03D0224-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse D Dimer ELISA kit
E03D0224-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse D Dimer ELISA kit
E03D0224-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig D Dimer ELISA kit
E07D0224-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig D Dimer ELISA kit
E07D0224-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig D Dimer ELISA kit
E07D0224-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat D Dimer ELISA kit
E06D0224-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat D Dimer ELISA kit
E06D0224-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat D Dimer ELISA kit
E06D0224-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog D Dimer ELISA kit
E08D0224-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog D Dimer ELISA kit
E08D0224-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog D Dimer ELISA kit
E08D0224-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

D-Dimer (D2D) Polyclonal Antibody (Human), APC
4-PAA506Hu01-APC	Cloud-Clone	EUR 301.00 EUR 2645.00 EUR 755.00 EUR 377.00 EUR 200.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with APC.

D-Dimer (D2D) Polyclonal Antibody (Human), Biotinylated
4-PAA506Hu01-Biotin	Cloud-Clone	EUR 279.00 EUR 1998.00 EUR 612.00 EUR 334.00 EUR 205.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with Biotin.

D-Dimer (D2D) Polyclonal Antibody (Human), Cy3
4-PAA506Hu01-Cy3	Cloud-Clone	EUR 360.00 EUR 3485.00 EUR 965.00 EUR 461.00 EUR 225.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with Cy3.

D-Dimer (D2D) Polyclonal Antibody (Human), FITC
4-PAA506Hu01-FITC	Cloud-Clone	EUR 261.00 EUR 2136.00 EUR 624.00 EUR 321.00 EUR 180.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with FITC.

D-Dimer (D2D) Polyclonal Antibody (Human), HRP
4-PAA506Hu01-HRP	Cloud-Clone	EUR 277.00 EUR 2309.00 EUR 671.00 EUR 343.00 EUR 190.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with HRP.

D-Dimer (D2D) Polyclonal Antibody (Human), PE
4-PAA506Hu01-PE	Cloud-Clone	EUR 261.00 EUR 2136.00 EUR 624.00 EUR 321.00 EUR 180.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with PE.

Human D-Dimer AssayLite Antibody (FITC Conjugate)
30191-05141	AssayPro	150 ug	EUR 428

Human D-Dimer AssayLite Antibody (RPE Conjugate)
30191-05151	AssayPro	150 ug	EUR 428

Human D-Dimer AssayLite Antibody (APC Conjugate)
30191-05161	AssayPro	150 ug	EUR 428

Human D-Dimer AssayLite Antibody (PerCP Conjugate)
30191-05171	AssayPro	150 ug	EUR 471

Mouse Anti Human D-Dimer Monoclonal Antibody
CABT-49328MH	Creative Diagnostics	0.2 mg	EUR 741

Pig D-Dimer, D2D ELISA Kit
CSB-E09399p-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Pig D-Dimer, D2D in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Pig D-Dimer, D2D ELISA Kit
1-CSB-E09399p	Cusabio	EUR 967.00 EUR 5925.00 EUR 3134.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Pig D-Dimer, D2D in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human D-Dimer, D2D ELISA Kit
CSB-E05175h-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human D-Dimer, D2D in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human D-Dimer, D2D ELISA Kit
1-CSB-E05175h	Cusabio	EUR 900.00 EUR 5476.00 EUR 2900.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human D-Dimer, D2D in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat D-Dimer, D2D ELISA Kit
CSB-E12984r-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rat D-Dimer, D2D in samples from serum, plasma, cell culture supernates, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat D-Dimer, D2D ELISA Kit
1-CSB-E12984r	Cusabio	EUR 900.00 EUR 5476.00 EUR 2900.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat D-Dimer, D2D in samples from serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse D-Dimer, D2D ELISA Kit
CSB-E13584m-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Mouse D-Dimer, D2D in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse D-Dimer, D2D ELISA Kit
1-CSB-E13584m	Cusabio	EUR 900.00 EUR 5476.00 EUR 2900.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse D-Dimer, D2D in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rabbit D-Dimer, D2D ELISA Kit
CSB-E10027Rb-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rabbit D-Dimer, D2D in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rabbit D-Dimer, D2D ELISA Kit
1-CSB-E10027Rb	Cusabio	EUR 967.00 EUR 5925.00 EUR 3134.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rabbit D-Dimer, D2D in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse D-Dimer (D2D)CLIA Kit
CCA506Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5804.88
Description: This is Competitive Chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D) in Plasma.

Mouse D-Dimer (D2D)CLIA Kit
CCA506Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 565.7
Description: This is Competitive Chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D) in Plasma.

Mouse D-Dimer (D2D)CLIA Kit
CCA506Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 765.28
Description: This is Competitive Chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D) in Plasma.

Mouse D-Dimer (D2D)CLIA Kit
CCA506Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3143.76
Description: This is Competitive Chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D) in Plasma.

Mouse D-Dimer (D2D) CLIA Kit
4-CCA506Mu	Cloud-Clone	EUR 5855.00 EUR 3144.00 EUR 766.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D)Plasma

Horse D-Dimer (D2D) ELISA Kit
CEA506Eq-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5215.83
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Horse D-Dimer (D2D) in Plasma.

Horse D-Dimer (D2D) ELISA Kit
CEA506Eq-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 517.19
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Horse D-Dimer (D2D) in Plasma.

Horse D-Dimer (D2D) ELISA Kit
CEA506Eq-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 695.98
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Horse D-Dimer (D2D) in Plasma.

Horse D-Dimer (D2D) ELISA Kit
CEA506Eq-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2831.91
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Horse D-Dimer (D2D) in Plasma.

Horse D-Dimer (D2D) ELISA Kit
4-CEA506Eq	Cloud-Clone	EUR 5266.00 EUR 2782.00 EUR 696.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Horse D-Dimer (D2D) in samples from Plasma with no significant corss-reactivity with analogues from other species.

Human D-Dimer (D2D) ELISA Kit
CEA506Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 3815.2
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human D-Dimer (D2D) in plasma.

Human D-Dimer (D2D) ELISA Kit
CEA506Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 401.84
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human D-Dimer (D2D) in plasma.

Human D-Dimer (D2D) ELISA Kit
CEA506Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 531.2
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human D-Dimer (D2D) in plasma.

Human D-Dimer (D2D) ELISA Kit
CEA506Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2090.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human D-Dimer (D2D) in plasma.

Human D-Dimer (D2D) ELISA Kit
4-CEA506Hu	Cloud-Clone	EUR 3866.00 EUR 2041.00 EUR 532.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species.

Mouse D-Dimer (D2D) ELISA Kit
CEA506Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 3919.92
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse D-Dimer (D2D) in plasma.

Mouse D-Dimer (D2D) ELISA Kit
CEA506Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 410.46
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse D-Dimer (D2D) in plasma.

Mouse D-Dimer (D2D) ELISA Kit
CEA506Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 543.52
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse D-Dimer (D2D) in plasma.

Mouse D-Dimer (D2D) ELISA Kit
CEA506Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2145.84
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse D-Dimer (D2D) in plasma.

Mouse D-Dimer (D2D) ELISA Kit
4-CEA506Mu	Cloud-Clone	EUR 3970.00 EUR 2096.00 EUR 544.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species.

Pig D-Dimer (D2D) ELISA Kit
CEA506Po-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5098.02
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Pig D-Dimer (D2D) in plasma.

Pig D-Dimer (D2D) ELISA Kit
CEA506Po-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 507.48
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Pig D-Dimer (D2D) in plasma.

Pig D-Dimer (D2D) ELISA Kit
CEA506Po-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 682.12
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Pig D-Dimer (D2D) in plasma.

Pig D-Dimer (D2D) ELISA Kit
CEA506Po-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2769.54
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Pig D-Dimer (D2D) in plasma.

Pig D-Dimer (D2D) ELISA Kit
4-CEA506Po	Cloud-Clone	EUR 5149.00 EUR 2720.00 EUR 683.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Pig D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species.

Rat D-Dimer (D2D) ELISA Kit
CEA506Ra-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4626.78
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat D-Dimer (D2D) in plasma.

Rat D-Dimer (D2D) ELISA Kit
CEA506Ra-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 468.68
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat D-Dimer (D2D) in plasma.

Rat D-Dimer (D2D) ELISA Kit
CEA506Ra-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 626.68
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat D-Dimer (D2D) in plasma.

Rat D-Dimer (D2D) ELISA Kit
CEA506Ra-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2520.06
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat D-Dimer (D2D) in plasma.

Rat D-Dimer (D2D) ELISA Kit
4-CEA506Ra	Cloud-Clone	EUR 4677.00 EUR 2471.00 EUR 627.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rat D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species.

Rabbit D-Dimer (D2D) ELISA Kit
CEA506Rb-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4626.78
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit D-Dimer (D2D) in Plasma.

Rabbit D-Dimer (D2D) ELISA Kit
CEA506Rb-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 468.68
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit D-Dimer (D2D) in Plasma.

Rabbit D-Dimer (D2D) ELISA Kit
CEA506Rb-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 626.68
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit D-Dimer (D2D) in Plasma.

Rabbit D-Dimer (D2D) ELISA Kit
CEA506Rb-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2520.06
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit D-Dimer (D2D) in Plasma.

Rabbit D-Dimer (D2D) ELISA Kit
4-CEA506Rb	Cloud-Clone	EUR 4677.00 EUR 2471.00 EUR 627.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rabbit D-Dimer (D2D) in samples from Plasma with no significant corss-reactivity with analogues from other species.

Human fibrin fragment D-dimer Mab
QZBFBDD13	Quickzyme	500 ug IgG	EUR 759

This exhibits the potential of canine in sustaining and spreading the an infection in Malaysia. As a result of occupational threat on account of frequent contact with canine and publicity to contaminated environments, canine handlers needs to be made conscious of the presence of this zoonotic illness.