Integrin Research The Martin Humphries Lab

Identification of critical chemical modifications and paratope mapping by size exclusion chromatography of stressed antibody-target complexes

Therapeutic proteins together with antibodies and Fc-fusion proteins bear numerous chemical modifications throughout cell tradition, purification, storage and in human circulation. They’re additionally uncovered to harsh situations throughout stress research, together with elevated temperature, extremes of pH, pressured oxidation, physiological pH, UV mild to evaluate the potential degradation pathways and suitability of strategies for detecting them. A few of these modifications are positioned on residues in binding areas, resulting in lack of binding and efficiency and categorised as vital high quality attributes.

At present, criticality of modifications is assessed by a laborious means of amassing antibody fractions from the mushy chromatography strategies ion change and hydrophobic interplay chromatography and characterizing the fractions one-by-one for efficiency and chemical modifications. Right here, we describe a technique for large-scale, parallel identification of all vital chemical modifications in a single experiment.

In step one, the antibody is harassed by one or a number of stress strategies. It’s then blended with goal protein and separated by size-exclusion chromatography (SEC) on sure antibody-target complicated and unbound antibody. Peptide mapping of fractions and statistical evaluation are carried out to establish modifications on amino acid residues that have an effect on binding.

To establish the modifications resulting in slight decreases in binding, aggressive SEC of antibody and antigen mixtures was developed and described in a companion research by Shi et al, the place goal protein is supplied at decrease stage, beneath the stoichiometry. The newly described methodology was efficiently correlated to crystallography for assessing criticality of chemical modifications and paratope mapping. It’s extra delicate to low-level modifications, higher streamlined and platform prepared.

Identification of vital chemical modifications by dimension exclusion chromatography of harassed antibody-target complexes with aggressive binding

Chemical modifications (attributes) within the binding areas of harassed therapeutic proteins might have an effect on binding to focus on and efficacy of therapeutic proteins. The tactic introduced right here describes the criticality evaluation of therapeutic antibody modifications by size-exclusion chromatography (SEC) of aggressive binding between a harassed antibody and its goal, human epidermal progress issue receptor-2 (HER2), adopted by SEC fractionation and peptide mapping characterization of sure and unbound antibodies.

When harassed antibody and its goal had been blended at a stoichiometric molar ratio of 1:2, solely antibody-receptor complicated eluted from SEC, indicating that binding was not decreased to interrupt the complicated. When a smaller quantity of the receptor was supplied (1:1), the antibody species with modifications decreasing binding eluted as unbound from SEC, whereas the antibody-receptor complicated eluted because the sure fraction. Peptide mapping revealed ratios of modifications between unbound and sure fractions.

Statistical evaluation after triplicate measurements (n = 3) indicated that heavy chain (HC) D102 isomerization and light-weight chain (LC) N30 deamidation had been four-fold greater in unbound fraction with excessive statistical significance. Though HC N55 deamidation and M107 oxidation had been additionally ample, they weren’t statistically completely different between unbound and sure.

Our findings agree with beforehand printed efficiency measurements of collected CEX fractions and the crystal construction of antibody and HER2. General, aggressive SEC of harassed antibody-receptor combination adopted by peptide mapping is a great tool in revealing vital residues and modifications concerned within the antibody-target binding, even when they elute as a posh from SEC when blended at 1:2 stoichiometric ratio.

Fast Dedication of Antibody-Antigen Affinity by Mass Photometry

Measurements of the specificity and affinity of antigen-antibody interactions are critically necessary for medical and analysis purposes. On this protocol, we describe the implementation of a brand new single-molecule method, mass photometry (MP), for this goal. MP is a label- and immobilization-free method that detects and quantifies molecular lots and populations of antibodies and antigen-antibody complexes on a single-molecule stage. MP analyzes the antigen-antibody pattern inside minutes, permitting for the exact dedication of the binding affinity and concurrently offering data on the stoichiometry and the oligomeric state of the proteins.

It is a easy and simple method that requires solely picomole portions of protein and no costly consumables. The identical process can be utilized to review protein-protein binding for proteins with a molecular mass bigger than 50 kDa. For multivalent protein interactions, the affinities of a number of binding websites might be obtained in a single measurement.

Nevertheless, the single-molecule mode of measurement and the shortage of labeling imposes some experimental limitations. This methodology offers the most effective outcomes when utilized to measurements of sub-micromolar interplay affinities, antigens with a molecular mass of 20 kDa or bigger, and comparatively pure protein samples. We additionally describe the process for performing the required becoming and calculation steps utilizing primary information evaluation software program.

Detection and distribution of anti-leptospiral antibody amongst canine and their handlers

The incidence of leptospirosis appears to be on the rise and could possibly be an alarming oblique indication of a world re-emergence. It’s a potential public well being menace when canine are alleged to be concerned within the transmission of leptospirosis by means of potential subclinical harbouring of Leptospira spp. and subsequent shedding into the atmosphere. This research aimed to detect anti-leptospiral antibodies amongst canine and their handlers utilizing the microscopic agglutination take a look at (MAT).

Blood samples from 266 apparently wholesome canine and 194 canine handlers had been collected at 4 working canine organisations and 4 canine shelters. Serum samples had been examined utilizing MAT in opposition to 20 leptospiral serovars with a cut-off titre >=1:100 (canine) and >=1:50 (canine handlers). Seventy canine (70/266; 26.3%) had been seropositive primarily in opposition to serovars Icterohaemorrhagiae, Ballum, Bataviae and Javanica (titres ranged: 1:100-1:800).

Sixty-seven canine handlers (67/194; 34.5%) had been seropositive primarily in opposition to serovars Grippotyphosa, Icterohaemorrhagiae and Malaysia (titres ranged: 1:50-1:200). Canines had been seropositive as a result of publicity, vaccination or lively an infection. Seropositive canine handlers may point out publicity or lively an infection.

Dog D Dimer ELISA kit
E08D0224-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig D Dimer ELISA kit
E07D0224-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig D Dimer ELISA kit
E07D0224-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig D Dimer ELISA kit
E07D0224-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat D Dimer ELISA kit
E06D0224-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat D Dimer ELISA kit
E06D0224-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat D Dimer ELISA kit
E06D0224-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse D Dimer ELISA kit
E03D0224-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse D Dimer ELISA kit
E03D0224-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse D Dimer ELISA kit
E03D0224-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human D Dimer ELISA kit
E01D0224-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human D Dimer ELISA kit
E01D0224-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human D Dimer ELISA kit
E01D0224-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit D Dimer ELISA kit
E04D0224-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit D Dimer ELISA kit
E04D0224-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit D Dimer ELISA kit
E04D0224-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey D Dimer ELISA kit
E09D0224-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey D Dimer ELISA kit
E09D0224-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey D Dimer ELISA kit
E09D0224-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit D- Dimer ELISA Kit
ELA-E0506Rb	Lifescience Market	96 Tests	EUR 1113.6

Porcine D- Dimer ELISA Kit
ELA-E0506p	Lifescience Market	96 Tests	EUR 1113.6

Guinea pig D Dimer ELISA kit
E05D0224-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig D Dimer ELISA kit
E05D0224-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig D Dimer ELISA kit
E05D0224-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

SLURP1 Dimer
pro-2295	ProSpec Tany	2µg	EUR 60
Description: Recombinant Human Secreted LY6PLAUR Domain Containing 1 Dimer

Pyrrolobenzodiazepine Dimer
ADC-P-132	Creative Biolabs	unit	Ask for price

Positive Control for Anti-Rat MEH Antibody
MEHCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CYP2C Antibody
P2CCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CYP2D Antibody
P2DCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CYP2B1 Antibody
P2B1PTCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CYP3A2 Antibody
P3A2PTCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Human CYP3A Antibody
P3ACON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Human CYP1B1 Antibody
PH1B1CON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Human CYP2E1 Antibody
P2E1PTCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Human CYP1A2 Antibody
P1A2CON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CHP2B1/2 Antibody
P2B12PTCON	Detroit R&D	100 uL	EUR 150

Octreotide trifluoroacetate salt (Dimer, Antiparallel)
H-7376.0500	Bachem	0.5mg	EUR 480
Description: Sum Formula: C98H132N20O20S4; CAS# [1926163-78-1] net

Octreotide trifluoroacetate salt (Dimer, Antiparallel)
H-7376.1000	Bachem	1.0mg	EUR 776.4
Description: Sum Formula: C98H132N20O20S4; CAS# [1926163-78-1] net

Positive Control for Anti-Rat CYP1A1/1A2 Antibody
P1ACON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Human Cytochrome b5 Antibody
Cb5CON	Detroit R&D	100 uL	EUR 150

D-Dimer Antibody
20-abx100081	Abbexa	EUR 343.20 EUR 159.60 EUR 828.00 EUR 427.20 EUR 292.80	100 ug 10 ug 1 mg 200 ug 50 ug

D-Dimer antibody
10-1155	Fitzgerald	250 ug	EUR 427.2
Description: Mouse monoclonal D-Dimer antibody

D-dimer antibody
10-1752	Fitzgerald	200 ul	EUR 651.6
Description: Mouse monoclonal D-dimer antibody

D-dimer antibody
10-1753	Fitzgerald	200 ul	EUR 651.6
Description: Mouse monoclonal D-dimer antibody

D-dimer antibody
10-2259	Fitzgerald	1 mg	EUR 418.8
Description: Mouse monoclonal D-dimer antibody

D-dimer antibody
10-7900	Fitzgerald	1 mg	EUR 642
Description: Mouse monoclonal D-dimer antibody

D-dimer antibody
10-D100A	Fitzgerald	1 mg	EUR 642
Description: Mouse monoclonal D-dimer antibody

D-Dimer antibody
10-D42A	Fitzgerald	1 mg	EUR 642
Description: Mouse monoclonal D-Dimer antibody

D-Dimer antibody
10-D42B	Fitzgerald	1 mg	EUR 642
Description: Mouse monoclonal D-Dimer antibody

D-Dimer antibody
10R-D100a	Fitzgerald	1 mg	EUR 642
Description: Mouse monoclonal D-Dimer antibody

D-Dimer antibody
10R-D100C	Fitzgerald	1 mg	EUR 642
Description: Mouse monoclonal D-Dimer antibody

D-Dimer Antibody
abx023583-1mg	Abbexa	1 mg	EUR 886.8

D-Dimer Antibody
abx023584-1mg	Abbexa	1 mg	EUR 886.8

D-Dimer Antibody
abx020755-1mg	Abbexa	1 mg	EUR 844.8

D-Dimer Antibody
abx020756-1mg	Abbexa	1 mg	EUR 844.8

D-Dimer Antibody
abx020759-1mg	Abbexa	1 mg	EUR 861.6

D-Dimer Antibody
abx020761-1mg	Abbexa	1 mg	EUR 886.8

D-Dimer Antibody
abx020762-1mg	Abbexa	1 mg	EUR 886.8

D-Dimer Antibody
abx020763-1mg	Abbexa	1 mg	EUR 886.8

D-Dimer Antibody
abx020764-1mg	Abbexa	1 mg	EUR 861.6

D-Dimer Antibody
abx020765-1mg	Abbexa	1 mg	EUR 886.8

D-Dimer Antibody
abx020767-1mg	Abbexa	1 mg	EUR 1479.6

D-Dimer Antibody
abx020769-1mg	Abbexa	1 mg	EUR 886.8

D-Dimer Antibody
abx020770-1mg	Abbexa	1 mg	EUR 886.8

D-Dimer Antibody
abx020771-1mg	Abbexa	1 mg	EUR 886.8

(Trp11,D-Phe15·16)-SDF-1 (7-16) (Dimer) (human, cat, mouse)
H-5876.0500	Bachem	0.5mg	EUR 1341.6
Description: Sum Formula: C134H180N34O26S2; CAS# [1926163-84-9] net

(Trp11,D-Phe15·16)-SDF-1 (7-16) (Dimer) (human, cat, mouse)
H-5876.1000	Bachem	1.0mg	EUR 2386.8
Description: Sum Formula: C134H180N34O26S2; CAS# [1926163-84-9] net

Anti-alpha/beta-Tubulin dimer Purified
11-445-C025	ExBio	0.025 mg	EUR 135.6

Anti-alpha/beta-Tubulin dimer Purified
11-445-C100	ExBio	0.1 mg	EUR 223.2

Anti-alpha/beta-Tubulin dimer Purified
11-446-C025	ExBio	0.025 mg	EUR 135.6

Anti-alpha/beta-Tubulin dimer Purified
11-446-C100	ExBio	0.1 mg	EUR 223.2

Anti-D-Dimer antibody
STJ400061	St John's Laboratory	1 mg	EUR 535.2
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400062	St John's Laboratory	1 mg	EUR 535.2
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400063	St John's Laboratory	1 mg	EUR 535.2
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400064	St John's Laboratory	1 mg	EUR 535.2
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400065	St John's Laboratory	1 mg	EUR 535.2
Description: D-Dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-Dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-Dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Anti-D-Dimer antibody
STJ400067	St John's Laboratory	1 mg	EUR 535.2
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT.

Chlorobis(Ethylene)Rhodium(I)Dimer
20-abx188647	Abbexa	EUR 427.20 EUR 1429.20 EUR 678.00 EUR 1045.20	100 mg 1 g 250 mg 500 mg

Human D-Dimer Antibody
30191-05111	AssayPro	150 ug	EUR 313.2

D-Dimer Antibody (Biotin)
abx020757-1mg	Abbexa	1 mg	EUR 1646.4

D-dimer antibody (biotin)
61-1009	Fitzgerald	100 ul	EUR 975.6
Description: Mouse monoclonal D-dimer antibody (Biotin)

D-Dimer Antibody (Biotin)
20-abx272516	Abbexa	EUR 477.60 EUR 276.00 EUR 1296.00 EUR 644.40 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

D-Dimer monoclonal antibody
10R-11476	Fitzgerald	1 mg	EUR 554.4
Description: Mouse anti-human D-Dimer monoclonal antibody

Efficiency Box--HRP conjugated secondary antibodies
EB-1	Detroit R&D	81 vials	EUR 193.2

D- Dimer Test Kit (Fluorescence Lateral Flow Immunoassay) , For Canine/Feline
HG020	EnoGene	10 Tests/Kit	EUR 70
Description: Contents: (1) 10 Tests/Kit  (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual

LIMITED QTY-Dinucleotide Standards Formamido Guanine Dimer d (PfpG) (50 µg)
0801210	Zeptometrix	50 µg	EUR 142.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Formamido Cytidine Dimer d (PfpC) (50 µg)
0801211	Zeptometrix	50 µg	EUR 142.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Formamido Thymine Dimer d (PfpT) (50 µg)
0801212	Zeptometrix	50 µg	EUR 142.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Formamido Adenine Dimer d (PfpA) (50 µg)
0801213	Zeptometrix	50 µg	EUR 142.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

(Cys26)-Amyloid b-Protein (1-40) (Dimer)
H-7418.0100	Bachem	0.1mg	EUR 471.6
Description: Sum Formula: C388H588N106O114S4; CAS# [1802087-75-7] net

(Cys26)-Amyloid b-Protein (1-40) (Dimer)
H-7418.0500	Bachem	0.5mg	EUR 762
Description: Sum Formula: C388H588N106O114S4; CAS# [1802087-75-7] net

LIMITED QTY-Dinucleotide Standards Thymine Glycol Cytidine Dimer d (TgpC) (50µg)
0801216	Zeptometrix	50µg	EUR 142.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

Octreotide trifluoroacetate salt (Dimer, Parallel)
H-7374.0500	Bachem	0.5mg	EUR 480
Description: Sum Formula: C98H132N20O20S4; CAS# [1926163-80-5] net

Octreotide trifluoroacetate salt (Dimer, Parallel)
H-7374.1000	Bachem	1.0mg	EUR 776.4
Description: Sum Formula: C98H132N20O20S4; CAS# [1926163-80-5] net

LIMITED QTY-Dinucleotide Standards Thymine Glycol Guanine Dimer d (TgpG) (50 µg)
0801214	Zeptometrix	50 µg	EUR 142.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Thymine Glycol Thymine Dimer d (TgpT) (50 µg)
0801215	Zeptometrix	50 µg	EUR 142.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

LIMITED QTY-Dinucleotide Standards Thymine Glycol Adenine Dimer d (TgpA) (50 µg)
0801217	Zeptometrix	50 µg	EUR 142.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

N-Acetylneuraminic Acid, dimer(α,2→8)
00640-46	NACALAI TESQUE	100MG	EUR 472.5

Dichloro(pentamethylcyclopentadienyl)iridium(iii)dimer
20-abx183980	Abbexa	EUR 777.60 EUR 427.20	1 g 250 mg

Dichloro(pentamethylcyclopentadienyl)rhodium(iii)dimer
abx183981-1g	Abbexa	1 g	EUR 910.8

Anti-D-Dimer Detection Antibody
A2090-100	Biovision	100 µg	EUR 405.6

Anti-D-Dimer Detection Antibody
A2091-100	Biovision	100 µg	EUR 405.6

Human D-dimer Antibody-BIOTIN
E64C00803	EnoGene	100ug/100ul	EUR 275
Description: Available in various conjugation types.

D-Dimer (D2D) Polyclonal Antibody
CAU27789-100ul	Biomatik Corporation	100ul	EUR 188.2

D-Dimer (D2D) Polyclonal Antibody
CAU27789-200ul	Biomatik Corporation	200ul	EUR 235.2

D-Dimer Monoclonal Antibody-Biotin
E63C00701b	EnoGene	100ug	EUR 225
Description: Available in various conjugation types.

Monoclonal Mouse anti-b actin
DRD96930	Detroit R&D	50 uL	EUR 336

Polyclonal Goat anti-mEH
MEH-1	Detroit R&D	50 uL	EUR 336

Polyclonal Goat anti-8 hydroxydeoxyguanosine
8OHDG	Detroit R&D	50 uL	EUR 368.4

Polyclonal Rabbit anti-sEH
SEH-1	Detroit R&D	50 uL	EUR 336

Rat FAP Protein, His Tag, active dimer (MALS verified)
FAP-R5246	ACROBIOSYSTEMS	100ug	EUR 2974.6
Description: Rat FAP, His Tag (FAP-R5246) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 761 (Accession # Q8R492-1).

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 336

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 336

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbit anti-GAPDH
DRD96417	Detroit R&D	50 uL	EUR 336

Efficiency Box refill (anti-goat)
EB-4	Detroit R&D	27 vials	EUR 121.2

Polyclonal anti-Cytochrome b5
Cb5	Detroit R&D	50 uL	EUR 336

Efficiency Box refill (anti-mouse)
EB-2	Detroit R&D	27 vials	EUR 121.2

Efficiency Box refill (anti-rabbit)
EB-3	Detroit R&D	27 vials	EUR 121.2

Positive Control for anti-GST a-forms: rat A1+A2 (Ya)
GSTCON1	Detroit R&D	100 uL	EUR 150

Positive Control for anti-GST a-forms: rat A3+A4 (Yc)
GSTCON11	Detroit R&D	100 uL	EUR 150

Positive Control for anti-GST m-forms: rat M1 (Yb)
GSTCON2	Detroit R&D	100 uL	EUR 150

Positive Control for anti-GST p-forms: rat P
GSTCON3	Detroit R&D	100 uL	EUR 150

Polyclonal Rabbit anti-Troponin I
TnI	Detroit R&D	50 uL	EUR 336

Monoclonal Anti-Cytochrome P450 2B1
P2B1PT	Detroit R&D	50 uL	EUR 336

Monoclonal Anti-Cytochrome P450 3A2
P3A2PT	Detroit R&D	50 uL	EUR 336

Monoclonal Anti-Cytochrome P450 1A1
P1A1PT	Detroit R&D	50 uL	EUR 336

Positive Control for anti-GST m-forms: rat M2 (Yb2)
GSTCON21	Detroit R&D	100 uL	EUR 150

Monoclonal Anti-Cytochrome P450 2B1/2B2
P2B12PT	Detroit R&D	50 uL	EUR 336

Monoclonal Anti-Cytochrome P450 2C11
P2C11PT	Detroit R&D	50 uL	EUR 336

Monoclonal Anti-Cytochrome P450 1A1/1A2
P1A12PT	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbit Anti-Cytochrome P450 2C
P2CR	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbit Anti-Cytochrome P450 2D
P2D	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbit Anti-Cytochrome P450 3A
P3A	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbit anti-phospho Akt (Thr308)
PAKT	Detroit R&D	50 uL	EUR 336

Human FAP Protein, Fc Tag, active dimer (MALS verified)
FAP-H5263	ACROBIOSYSTEMS	20ug	EUR 2931.8
Description: Human FAP, Fc Tag (FAP-H5263) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).

Polyclonal Rabbit Anti-Cytochrome P450 1B1
PH1B1	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbit Anti-Cytochrome P450 2D6
P2D6	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbit Anti-Cytochrome P450 2E1
P2E1PT	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbbit Anti-Cytochrome P450 1A2
PH1A2	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbit Anti-Cytochrome P450 3A23
P3A23R	Detroit R&D	50 uL	EUR 336

Polyclonal Rabbit Anti-Cytochrome P450 2C8/2C9/2C10
P2C	Detroit R&D	50 uL	EUR 336

Monoclonal Mouse anti-glycosylated p53 (Ser149)
GP53	Detroit R&D	50 uL	EUR 505.2

Human FAP Protein, His Tag, active dimer (MALS verified)
FAP-H5244	ACROBIOSYSTEMS	20ug	EUR 321
Description: Human FAP, His Tag (FAP-H5244) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).

Mouse FAP Protein, His Tag, active dimer (MALS verified)
FAP-M52H3	ACROBIOSYSTEMS	100ug	EUR 2974.6
Description: Mouse FAP, His Tag (FAP-M52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 761 (Accession # P97321).

D-Dimer (D2D) Polyclonal Antibody (Human)
4-PAA506Hu01	Cloud-Clone	EUR 260.40 EUR 2457.60 EUR 624.00 EUR 321.60 EUR 241.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D)

Mouse CD28 Protein, His Tag, active dimer (MALS verified)
CD8-M52H6	ACROBIOSYSTEMS	1mg	EUR 331.7
Description: Mouse CD28, His Tag (CD8-M52H6) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Lys 149 (Accession # P31041-1).

Human D-Dimer Antibody (Biotin Conjugate)
30191-05121	AssayPro	150 ug	EUR 442.8

Human TIGIT Protein, His Tag, active dimer (MALS verified)
TIT-H52H5	ACROBIOSYSTEMS	100ug	EUR 374.5
Description: Human TIGIT, His Tag (TIT-H52H5) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).

Recombinant Human Follicle-Stimulating Hormone α/β Dimer
CM28-10ug	Novoprotein	10ug	EUR 242.4
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Follicle-Stimulating Hormone α/β Dimer
CM28-1mg	Novoprotein	1mg	EUR 2983.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Follicle-Stimulating Hormone α/β Dimer
CM28-500ug	Novoprotein	500ug	EUR 2106
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Follicle-Stimulating Hormone α/β Dimer
CM28-50ug	Novoprotein	50ug	EUR 595.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Cynomolgus FAP Protein, His Tag, active dimer (MALS verified)
FAP-C52H3	ACROBIOSYSTEMS	100ug	EUR 2974.6
Description: Cynomolgus FAP, His Tag (FAP-C52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # XP_005573377.1).

Mouse Anti Human D-Dimer Monoclonal Antibody
CABT-49328MH	Creative Diagnostics	0.2 mg	EUR 889.2

D-Dimer (D2D) Polyclonal Antibody (Human), PE
4-PAA506Hu01-PE	Cloud-Clone	EUR 313.20 EUR 2563.20 EUR 748.80 EUR 385.20 EUR 216.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with PE.

D-Dimer (D2D) Polyclonal Antibody (Human), APC
4-PAA506Hu01-APC	Cloud-Clone	EUR 361.20 EUR 3174.00 EUR 906.00 EUR 452.40 EUR 240.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with APC.

D-Dimer (D2D) Polyclonal Antibody (Human), Cy3
4-PAA506Hu01-Cy3	Cloud-Clone	EUR 432.00 EUR 4182.00 EUR 1158.00 EUR 553.20 EUR 270.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with Cy3.

D-Dimer (D2D) Polyclonal Antibody (Human), HRP
4-PAA506Hu01-HRP	Cloud-Clone	EUR 332.40 EUR 2770.80 EUR 805.20 EUR 411.60 EUR 228.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with HRP.

D-Dimer (D2D) Polyclonal Antibody (Human), FITC
4-PAA506Hu01-FITC	Cloud-Clone	EUR 313.20 EUR 2563.20 EUR 748.80 EUR 385.20 EUR 216.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with FITC.

Human CTLA-4 / CD152 Protein, His Tag, active dimer (MALS verified)
CT4-H52H9	ACROBIOSYSTEMS	100ug	EUR 2354
Description: Human CTLA-4, His Tag (CT4-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # P16410-1).

Human D-Dimer AssayLite Antibody (RPE Conjugate)
30191-05151	AssayPro	150 ug	EUR 513.6

Human D-Dimer AssayLite Antibody (APC Conjugate)
30191-05161	AssayPro	150 ug	EUR 513.6

Human D-Dimer AssayLite Antibody (FITC Conjugate)
30191-05141	AssayPro	150 ug	EUR 513.6

Human D-Dimer AssayLite Antibody (PerCP Conjugate)
30191-05171	AssayPro	150 ug	EUR 565.2

15-HETE Hypertension ELISA Kit
15H1	Detroit R&D	1 Kit	EUR 368.4

This exhibits the potential of canine in sustaining and spreading the an infection in Malaysia. As a result of occupational threat on account of frequent contact with canine and publicity to contaminated environments, canine handlers needs to be made conscious of the presence of this zoonotic illness.