Identification of critical chemical modifications and paratope mapping by size exclusion chromatography of stressed antibody-target complexes
Therapeutic proteins together with antibodies and Fc-fusion proteins bear numerous chemical modifications throughout cell tradition, purification, storage and in human circulation. They’re additionally uncovered to harsh situations throughout stress research, together with elevated temperature, extremes of pH, pressured oxidation, physiological pH, UV mild to evaluate the potential degradation pathways and suitability of strategies for detecting them. A few of these modifications are positioned on residues in binding areas, resulting in lack of binding and efficiency and categorised as vital high quality attributes.
At present, criticality of modifications is assessed by a laborious means of amassing antibody fractions from the mushy chromatography strategies ion change and hydrophobic interplay chromatography and characterizing the fractions one-by-one for efficiency and chemical modifications. Right here, we describe a technique for large-scale, parallel identification of all vital chemical modifications in a single experiment.
In step one, the antibody is harassed by one or a number of stress strategies. It’s then blended with goal protein and separated by size-exclusion chromatography (SEC) on sure antibody-target complicated and unbound antibody. Peptide mapping of fractions and statistical evaluation are carried out to establish modifications on amino acid residues that have an effect on binding.
To establish the modifications resulting in slight decreases in binding, aggressive SEC of antibody and antigen mixtures was developed and described in a companion research by Shi et al, the place goal protein is supplied at decrease stage, beneath the stoichiometry. The newly described methodology was efficiently correlated to crystallography for assessing criticality of chemical modifications and paratope mapping. It’s extra delicate to low-level modifications, higher streamlined and platform prepared.
Identification of vital chemical modifications by dimension exclusion chromatography of harassed antibody-target complexes with aggressive binding
Chemical modifications (attributes) within the binding areas of harassed therapeutic proteins might have an effect on binding to focus on and efficacy of therapeutic proteins. The tactic introduced right here describes the criticality evaluation of therapeutic antibody modifications by size-exclusion chromatography (SEC) of aggressive binding between a harassed antibody and its goal, human epidermal progress issue receptor-2 (HER2), adopted by SEC fractionation and peptide mapping characterization of sure and unbound antibodies.
When harassed antibody and its goal had been blended at a stoichiometric molar ratio of 1:2, solely antibody-receptor complicated eluted from SEC, indicating that binding was not decreased to interrupt the complicated. When a smaller quantity of the receptor was supplied (1:1), the antibody species with modifications decreasing binding eluted as unbound from SEC, whereas the antibody-receptor complicated eluted because the sure fraction. Peptide mapping revealed ratios of modifications between unbound and sure fractions.
Statistical evaluation after triplicate measurements (n = 3) indicated that heavy chain (HC) D102 isomerization and light-weight chain (LC) N30 deamidation had been four-fold greater in unbound fraction with excessive statistical significance. Though HC N55 deamidation and M107 oxidation had been additionally ample, they weren’t statistically completely different between unbound and sure.
Our findings agree with beforehand printed efficiency measurements of collected CEX fractions and the crystal construction of antibody and HER2. General, aggressive SEC of harassed antibody-receptor combination adopted by peptide mapping is a great tool in revealing vital residues and modifications concerned within the antibody-target binding, even when they elute as a posh from SEC when blended at 1:2 stoichiometric ratio.
Fast Dedication of Antibody-Antigen Affinity by Mass Photometry
Measurements of the specificity and affinity of antigen-antibody interactions are critically necessary for medical and analysis purposes. On this protocol, we describe the implementation of a brand new single-molecule method, mass photometry (MP), for this goal. MP is a label- and immobilization-free method that detects and quantifies molecular lots and populations of antibodies and antigen-antibody complexes on a single-molecule stage. MP analyzes the antigen-antibody pattern inside minutes, permitting for the exact dedication of the binding affinity and concurrently offering data on the stoichiometry and the oligomeric state of the proteins.

It is a easy and simple method that requires solely picomole portions of protein and no costly consumables. The identical process can be utilized to review protein-protein binding for proteins with a molecular mass bigger than 50 kDa. For multivalent protein interactions, the affinities of a number of binding websites might be obtained in a single measurement.
Nevertheless, the single-molecule mode of measurement and the shortage of labeling imposes some experimental limitations. This methodology offers the most effective outcomes when utilized to measurements of sub-micromolar interplay affinities, antigens with a molecular mass of 20 kDa or bigger, and comparatively pure protein samples. We additionally describe the process for performing the required becoming and calculation steps utilizing primary information evaluation software program.
Detection and distribution of anti-leptospiral antibody amongst canine and their handlers
The incidence of leptospirosis appears to be on the rise and could possibly be an alarming oblique indication of a world re-emergence. It’s a potential public well being menace when canine are alleged to be concerned within the transmission of leptospirosis by means of potential subclinical harbouring of Leptospira spp. and subsequent shedding into the atmosphere. This research aimed to detect anti-leptospiral antibodies amongst canine and their handlers utilizing the microscopic agglutination take a look at (MAT).
Blood samples from 266 apparently wholesome canine and 194 canine handlers had been collected at 4 working canine organisations and 4 canine shelters. Serum samples had been examined utilizing MAT in opposition to 20 leptospiral serovars with a cut-off titre >=1:100 (canine) and >=1:50 (canine handlers). Seventy canine (70/266; 26.3%) had been seropositive primarily in opposition to serovars Icterohaemorrhagiae, Ballum, Bataviae and Javanica (titres ranged: 1:100-1:800).
Sixty-seven canine handlers (67/194; 34.5%) had been seropositive primarily in opposition to serovars Grippotyphosa, Icterohaemorrhagiae and Malaysia (titres ranged: 1:50-1:200). Canines had been seropositive as a result of publicity, vaccination or lively an infection. Seropositive canine handlers may point out publicity or lively an infection.
D-dimer antibody |
10-1752 |
Fitzgerald |
200 ul |
EUR 651.6 |
Description: Mouse monoclonal D-dimer antibody |
D-dimer antibody |
10-1753 |
Fitzgerald |
200 ul |
EUR 651.6 |
Description: Mouse monoclonal D-dimer antibody |
D-dimer antibody |
10-2259 |
Fitzgerald |
1 mg |
EUR 418.8 |
Description: Mouse monoclonal D-dimer antibody |
D-dimer antibody |
10-7900 |
Fitzgerald |
1 mg |
EUR 642 |
Description: Mouse monoclonal D-dimer antibody |
D-dimer antibody |
10-D100A |
Fitzgerald |
1 mg |
EUR 642 |
Description: Mouse monoclonal D-dimer antibody |
D-Dimer antibody |
10-D42A |
Fitzgerald |
1 mg |
EUR 642 |
Description: Mouse monoclonal D-Dimer antibody |
D-Dimer antibody |
10-D42B |
Fitzgerald |
1 mg |
EUR 642 |
Description: Mouse monoclonal D-Dimer antibody |
D-Dimer antibody |
10R-D100a |
Fitzgerald |
1 mg |
EUR 642 |
Description: Mouse monoclonal D-Dimer antibody |
D-Dimer antibody |
10R-D100C |
Fitzgerald |
1 mg |
EUR 642 |
Description: Mouse monoclonal D-Dimer antibody |
D-Dimer Antibody |
20-abx100081 |
Abbexa |
-
EUR 343.20
-
EUR 159.60
-
EUR 828.00
-
EUR 427.20
-
EUR 292.80
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
D-Dimer Antibody |
abx020755-1mg |
Abbexa |
1 mg |
EUR 844.8 |
|
D-Dimer Antibody |
abx020756-1mg |
Abbexa |
1 mg |
EUR 844.8 |
|
D-Dimer Antibody |
abx020759-1mg |
Abbexa |
1 mg |
EUR 861.6 |
|
D-Dimer Antibody |
abx020761-1mg |
Abbexa |
1 mg |
EUR 886.8 |
|
D-Dimer Antibody |
abx020762-1mg |
Abbexa |
1 mg |
EUR 886.8 |
|
D-Dimer Antibody |
abx020763-1mg |
Abbexa |
1 mg |
EUR 886.8 |
|
D-Dimer Antibody |
abx020764-1mg |
Abbexa |
1 mg |
EUR 861.6 |
|
D-Dimer Antibody |
abx020765-1mg |
Abbexa |
1 mg |
EUR 886.8 |
|
D-Dimer Antibody |
abx020767-1mg |
Abbexa |
1 mg |
EUR 1479.6 |
|
D-Dimer Antibody |
abx020769-1mg |
Abbexa |
1 mg |
EUR 886.8 |
|
D-Dimer Antibody |
abx020770-1mg |
Abbexa |
1 mg |
EUR 886.8 |
|
D-Dimer Antibody |
abx020771-1mg |
Abbexa |
1 mg |
EUR 886.8 |
|
D-Dimer Antibody |
abx023583-1mg |
Abbexa |
1 mg |
EUR 886.8 |
|
D-Dimer Antibody |
abx023584-1mg |
Abbexa |
1 mg |
EUR 886.8 |
|
D-dimer antibody (biotin) |
61-1009 |
Fitzgerald |
100 ul |
EUR 975.6 |
Description: Mouse monoclonal D-dimer antibody (Biotin) |
D-Dimer monoclonal antibody |
10R-11476 |
Fitzgerald |
1 mg |
EUR 554.4 |
Description: Mouse anti-human D-Dimer monoclonal antibody |
Human D-Dimer Antibody |
30191-05111 |
AssayPro |
150 ug |
EUR 313.2 |
D-Dimer Antibody (Biotin) |
abx020757-1mg |
Abbexa |
1 mg |
EUR 1646.4 |
|
D-Dimer Antibody (Biotin) |
20-abx272516 |
Abbexa |
-
EUR 477.60
-
EUR 276.00
-
EUR 1296.00
-
EUR 644.40
-
EUR 376.80
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Anti-D-Dimer antibody |
STJ400061 |
St John's Laboratory |
1 mg |
EUR 535.2 |
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT. |
Anti-D-Dimer antibody |
STJ400062 |
St John's Laboratory |
1 mg |
EUR 535.2 |
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT. |
Anti-D-Dimer antibody |
STJ400063 |
St John's Laboratory |
1 mg |
EUR 535.2 |
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT. |
Anti-D-Dimer antibody |
STJ400064 |
St John's Laboratory |
1 mg |
EUR 535.2 |
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT. |
Anti-D-Dimer antibody |
STJ400065 |
St John's Laboratory |
1 mg |
EUR 535.2 |
Description: D-Dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-Dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-Dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT. |
Anti-D-Dimer antibody |
STJ400067 |
St John's Laboratory |
1 mg |
EUR 535.2 |
Description: D-dimer (DD) is a fibrin degradation product created during fibrinolysis when plasmin degrades the fibrin clot. In clinical diagnostics, D-dimer test can be used to exclude deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). D-dimer is also valuable for monitoring patients during and after anticoagulant treatment for recurrent DVT. |
D-Dimer protein |
30R-AD001 |
Fitzgerald |
1 mg |
EUR 2050.8 |
Description: Purified native Human D-Dimer protein |
D-Dimer protein |
30-1099S |
Fitzgerald |
100 ug |
EUR 292.8 |
Description: Purified native Human D-Dimer protein |
D Dimer protein |
30-1978 |
Fitzgerald |
1 mg |
EUR 1094.4 |
Description: Human D Dimer protein |
D-Dimer Protein |
abx061440-1mg |
Abbexa |
1 mg |
EUR 1629.6 |
|
D-Dimer Protein |
abx069809-1mg |
Abbexa |
1 mg |
EUR 1646.4 |
|
D-dimer Antigen |
E64C00801 |
EnoGene |
1mg |
EUR 2010 |
D-dimer Antigen |
E64C00802 |
EnoGene |
100ug |
EUR 411.6 |
Anti-D-Dimer Detection Antibody |
A2090-100 |
Biovision |
100 µg |
EUR 405.6 |
Anti-D-Dimer Detection Antibody |
A2091-100 |
Biovision |
100 µg |
EUR 405.6 |
Human D-Dimer Protein |
20-abx060114 |
Abbexa |
|
|
|
Human D-Dimer Protein |
abx060234-1mg |
Abbexa |
1 mg |
EUR 678 |
|
Human D-Dimer Protein |
abx060717-100ug |
Abbexa |
100 ug |
EUR 1554 |
|
D-Dimer Assay Kit |
abx098424-Hitachi7020R145ml1R215ml1 |
Abbexa |
Hitachi 7020; R1:45ml×1 R2:15ml×1 |
EUR 2408.4 |
|
D-Dimer Assay Kit |
abx098424-Hitachi7060R145ml1R215ml1 |
Abbexa |
Hitachi 7060; R1:45ml×1 R2:15ml×1 |
EUR 2408.4 |
|
D-Dimer Assay Kit |
abx098424-Hitachi7170R130ml1R210ml1 |
Abbexa |
Hitachi 7170; R1:30ml×1 R2:10ml×1 |
EUR 1675.2 |
|
D-Dimer Assay Kit |
abx098424-Hitachi7170R145ml1R215ml1 |
Abbexa |
Hitachi 7170; R1:45ml×1 R2:15ml×1 |
EUR 2408.4 |
|
D-Dimer Assay Kit |
abx098424-Toshiba40R145ml1R215ml1 |
Abbexa |
Toshiba 40; R1:45ml×1 R2:15ml×1 |
EUR 2408.4 |
|
D-Dimer (Capture Ab) |
abx019235-1mg |
Abbexa |
1 mg |
EUR 644.4 |
|
D-Dimer (Capture Ab) |
abx019236-1mg |
Abbexa |
1 mg |
EUR 644.4 |
|
D-Dimer (Detection Ab) |
abx019237-1mg |
Abbexa |
1 mg |
EUR 644.4 |
|
Human D-Dimer Protein |
abx670031-02mg |
Abbexa |
0.2 mg |
EUR 910.8 |
|
D-Dimer, Human Plasma |
P1434-100 |
Biovision |
|
EUR 248.4 |
D-Dimer, Human Plasma |
P1434-1000 |
Biovision |
|
EUR 724.8 |
Human D-Dimer Antibody (Biotin Conjugate) |
30191-05121 |
AssayPro |
150 ug |
EUR 442.8 |
D-Dimer (D2D) Polyclonal Antibody (Human) |
4-PAA506Hu01 |
Cloud-Clone |
-
EUR 260.40
-
EUR 2457.60
-
EUR 624.00
-
EUR 321.60
-
EUR 241.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D) |
D-Dimer protein (> 98% pure) |
30-1021 |
Fitzgerald |
100 ug |
EUR 807.6 |
Description: Purified native Human D-Dimer protein |
Horse D-Dimer ELISA Kit |
20-abx150441 |
Abbexa |
-
EUR 8853.60
-
EUR 4719.60
-
EUR 1093.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Human D-Dimer ELISA Kit |
20-abx151266 |
Abbexa |
-
EUR 7407.60
-
EUR 3949.20
-
EUR 924.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Pig D-Dimer ELISA Kit |
20-abx154909 |
Abbexa |
-
EUR 9174.00
-
EUR 4888.80
-
EUR 1130.40
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rabbit D-Dimer ELISA Kit |
20-abx155054 |
Abbexa |
-
EUR 8684.40
-
EUR 4626.00
-
EUR 1074.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rat D-Dimer ELISA Kit |
20-abx155431 |
Abbexa |
-
EUR 8684.40
-
EUR 4626.00
-
EUR 1074.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Human D-Dimer Protein (OVA) |
20-abx165525 |
Abbexa |
-
EUR 693.60
-
EUR 309.60
-
EUR 2064.00
-
EUR 828.00
-
EUR 510.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Pig D-Dimer ELISA Kit |
abx051362-96tests |
Abbexa |
96 tests |
EUR 990 |
|
Human D-Dimer CLIA Kit |
abx196592-96tests |
Abbexa |
96 tests |
EUR 990 |
|
Human D-Dimer ELISA Kit |
abx253171-96tests |
Abbexa |
96 tests |
EUR 904.8 |
|
Monkey D-Dimer ELISA Kit |
abx353336-96tests |
Abbexa |
96 tests |
EUR 990 |
|
Dog D-Dimer ELISA Kit |
abx354632-96tests |
Abbexa |
96 tests |
EUR 1111.2 |
|
Chicken D-Dimer ELISA Kit |
abx354774-96tests |
Abbexa |
96 tests |
EUR 990 |
|
Rat D-Dimer CLIA Kit |
20-abx490570 |
Abbexa |
-
EUR 9567.60
-
EUR 5095.20
-
EUR 1177.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rat D-Dimer ELISA Kit |
abx575948-96tests |
Abbexa |
96 tests |
EUR 943.2 |
|
Mouse D-Dimer Protein (OVA) |
20-abx655872 |
Abbexa |
-
EUR 693.60
-
EUR 309.60
-
EUR 2064.00
-
EUR 828.00
-
EUR 510.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Rat D-Dimer ELISA Kit |
abx256862-96tests |
Abbexa |
96 tests |
EUR 943.2 |
|
Rabbit D-Dimer ELISA Kit |
abx257004-96tests |
Abbexa |
96 tests |
EUR 943.2 |
|
Pig D-Dimer ELISA Kit |
abx257608-96tests |
Abbexa |
96 tests |
EUR 990 |
|
Mouse D-Dimer ELISA Kit |
20-abx258705 |
Abbexa |
-
EUR 8853.60
-
EUR 4719.60
-
EUR 1093.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rat D-Dimer ELISA Kit |
20-abx258706 |
Abbexa |
-
EUR 7970.40
-
EUR 4250.40
-
EUR 990.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Horse D-Dimer CLIA Kit |
20-abx490143 |
Abbexa |
-
EUR 10282.80
-
EUR 5472.00
-
EUR 1262.40
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Human D-Dimer CLIA Kit |
20-abx490144 |
Abbexa |
-
EUR 8853.60
-
EUR 4719.60
-
EUR 1093.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Pig D-Dimer CLIA Kit |
20-abx490145 |
Abbexa |
-
EUR 10282.80
-
EUR 5472.00
-
EUR 1262.40
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rat D-Dimer CLIA Kit |
20-abx490146 |
Abbexa |
-
EUR 10282.80
-
EUR 5472.00
-
EUR 1262.40
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rabbit D-Dimer CLIA Kit |
20-abx490147 |
Abbexa |
-
EUR 10282.80
-
EUR 5472.00
-
EUR 1262.40
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
OVA Conjugated D-Dimer (D2D) |
4-CPA506Hu21 |
Cloud-Clone |
-
EUR 496.32
-
EUR 256.80
-
EUR 1531.20
-
EUR 590.40
-
EUR 1060.80
-
EUR 408.00
-
EUR 3648.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human D-Dimer expressed in: Protein conjugation |
Human D-DIMER ELISA Kit |
DEIADD2911 |
Creative Diagnostics |
96T |
EUR 752.4 |
Description: The CD Human D-DIMER ELISA Kit is an enzyme-linked immunosorbent assay for measuring Human DDIMER in serum, plasma, and cell culture media. |
Goat D Dimer ELISA kit |
E06D0224-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat D Dimer ELISA kit |
E06D0224-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat D Dimer ELISA kit |
E06D0224-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Goat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit D Dimer ELISA kit |
E04D0224-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit D Dimer ELISA kit |
E04D0224-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit D Dimer ELISA kit |
E04D0224-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse D Dimer ELISA kit |
E03D0224-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse D Dimer ELISA kit |
E03D0224-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse D Dimer ELISA kit |
E03D0224-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog D Dimer ELISA kit |
E08D0224-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog D Dimer ELISA kit |
E08D0224-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog D Dimer ELISA kit |
E08D0224-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Canine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey D Dimer ELISA kit |
E09D0224-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey D Dimer ELISA kit |
E09D0224-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey D Dimer ELISA kit |
E09D0224-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat D Dimer ELISA kit |
E02D0224-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Rat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat D Dimer ELISA kit |
E02D0224-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Rat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat D Dimer ELISA kit |
E02D0224-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Rat D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human D Dimer ELISA kit |
E01D0224-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human D Dimer ELISA kit |
E01D0224-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human D Dimer ELISA kit |
E01D0224-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Human D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig D Dimer ELISA kit |
E07D0224-192T |
BlueGene |
192 tests |
EUR 1524 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig D Dimer ELISA kit |
E07D0224-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig D Dimer ELISA kit |
E07D0224-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine D Dimer in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human D-dimer ELISA Kit |
PRB-5048 |
Cell Biolabs |
96 assays |
EUR 686.4 |
Description: Cell Biolabs? Human D-dimer ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human D-dimer in plasma, serum or other biological fluid samples. The kit has a detection sensitivity limit of 150 pg/mL human D-dimer. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. |
Human D-dimer ELISA Kit |
PRB-5048-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2739.6 |
Description: Cell Biolabs? Human D-dimer ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human D-dimer in plasma, serum or other biological fluid samples. The kit has a detection sensitivity limit of 150 pg/mL human D-dimer. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. |
Human D-Dimer AssayLite Antibody (FITC Conjugate) |
30191-05141 |
AssayPro |
150 ug |
EUR 513.6 |
Human D-Dimer AssayLite Antibody (RPE Conjugate) |
30191-05151 |
AssayPro |
150 ug |
EUR 513.6 |
Human D-Dimer AssayLite Antibody (APC Conjugate) |
30191-05161 |
AssayPro |
150 ug |
EUR 513.6 |
Human D-Dimer AssayLite Antibody (PerCP Conjugate) |
30191-05171 |
AssayPro |
150 ug |
EUR 565.2 |
D-Dimer (D2D) Polyclonal Antibody (Human), APC |
4-PAA506Hu01-APC |
Cloud-Clone |
-
EUR 361.20
-
EUR 3174.00
-
EUR 906.00
-
EUR 452.40
-
EUR 240.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with APC. |
D-Dimer (D2D) Polyclonal Antibody (Human), Biotinylated |
4-PAA506Hu01-Biotin |
Cloud-Clone |
-
EUR 334.80
-
EUR 2397.60
-
EUR 734.40
-
EUR 400.80
-
EUR 246.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with Biotin. |
D-Dimer (D2D) Polyclonal Antibody (Human), Cy3 |
4-PAA506Hu01-Cy3 |
Cloud-Clone |
-
EUR 432.00
-
EUR 4182.00
-
EUR 1158.00
-
EUR 553.20
-
EUR 270.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with Cy3. |
D-Dimer (D2D) Polyclonal Antibody (Human), FITC |
4-PAA506Hu01-FITC |
Cloud-Clone |
-
EUR 313.20
-
EUR 2563.20
-
EUR 748.80
-
EUR 385.20
-
EUR 216.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with FITC. |
D-Dimer (D2D) Polyclonal Antibody (Human), HRP |
4-PAA506Hu01-HRP |
Cloud-Clone |
-
EUR 332.40
-
EUR 2770.80
-
EUR 805.20
-
EUR 411.60
-
EUR 228.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with HRP. |
D-Dimer (D2D) Polyclonal Antibody (Human), PE |
4-PAA506Hu01-PE |
Cloud-Clone |
-
EUR 313.20
-
EUR 2563.20
-
EUR 748.80
-
EUR 385.20
-
EUR 216.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human D-Dimer (D2D). This antibody is labeled with PE. |
D-Dimer (Up-converting Phosphor Technology) |
abx095256-80Units |
Abbexa |
80 Units |
EUR 1579.2 |
|
Guinea pig D-Dimer ELISA Kit |
abx354785-96tests |
Abbexa |
96 tests |
EUR 990 |
|
Human D-Dimer,D2D ELISA Kit |
201-12-1114 |
SunredBio |
96 tests |
EUR 528 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Mouse D-Dimer (D2D)CLIA Kit |
CCA506Mu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6965.86 |
|
Description: This is Competitive Chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D) in Plasma. |
Mouse D-Dimer (D2D)CLIA Kit |
CCA506Mu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 678.84 |
|
Description: This is Competitive Chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D) in Plasma. |
Mouse D-Dimer (D2D)CLIA Kit |
CCA506Mu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 918.34 |
|
Description: This is Competitive Chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D) in Plasma. |
Mouse D-Dimer (D2D)CLIA Kit |
CCA506Mu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3772.51 |
|
Description: This is Competitive Chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D) in Plasma. |
Mouse D-Dimer (D2D) CLIA Kit |
4-CCA506Mu |
Cloud-Clone |
-
EUR 7026.00
-
EUR 3772.80
-
EUR 919.20
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Mouse D-Dimer (D2D)Plasma |
Horse D-Dimer (D2D) ELISA Kit |
CEA506Eq-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6259 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Horse D-Dimer (D2D) in Plasma. |
Horse D-Dimer (D2D) ELISA Kit |
CEA506Eq-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 620.63 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Horse D-Dimer (D2D) in Plasma. |
Horse D-Dimer (D2D) ELISA Kit |
CEA506Eq-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 835.18 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Horse D-Dimer (D2D) in Plasma. |
Horse D-Dimer (D2D) ELISA Kit |
CEA506Eq-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3398.29 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Horse D-Dimer (D2D) in Plasma. |
Horse D-Dimer (D2D) ELISA Kit |
4-CEA506Eq |
Cloud-Clone |
-
EUR 6319.20
-
EUR 3338.40
-
EUR 835.20
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Horse D-Dimer (D2D) in samples from Plasma with no significant corss-reactivity with analogues from other species. |
Human D-Dimer (D2D) ELISA Kit |
CEA506Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4578.24 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human D-Dimer (D2D) in plasma. |
Human D-Dimer (D2D) ELISA Kit |
CEA506Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 482.21 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human D-Dimer (D2D) in plasma. |
Human D-Dimer (D2D) ELISA Kit |
CEA506Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 637.44 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human D-Dimer (D2D) in plasma. |
Human D-Dimer (D2D) ELISA Kit |
CEA506Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2508.48 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human D-Dimer (D2D) in plasma. |
Human D-Dimer (D2D) ELISA Kit |
4-CEA506Hu |
Cloud-Clone |
-
EUR 4639.20
-
EUR 2449.20
-
EUR 638.40
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species. |
Mouse D-Dimer (D2D) ELISA Kit |
CEA506Mu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4703.9 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse D-Dimer (D2D) in plasma. |
Mouse D-Dimer (D2D) ELISA Kit |
CEA506Mu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 492.55 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse D-Dimer (D2D) in plasma. |
Mouse D-Dimer (D2D) ELISA Kit |
CEA506Mu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 652.22 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse D-Dimer (D2D) in plasma. |
Mouse D-Dimer (D2D) ELISA Kit |
CEA506Mu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2575.01 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse D-Dimer (D2D) in plasma. |
Mouse D-Dimer (D2D) ELISA Kit |
4-CEA506Mu |
Cloud-Clone |
-
EUR 4764.00
-
EUR 2515.20
-
EUR 652.80
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species. |
Pig D-Dimer (D2D) ELISA Kit |
CEA506Po-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6117.62 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Pig D-Dimer (D2D) in plasma. |
Pig D-Dimer (D2D) ELISA Kit |
CEA506Po-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 608.98 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Pig D-Dimer (D2D) in plasma. |
Pig D-Dimer (D2D) ELISA Kit |
CEA506Po-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 818.54 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Pig D-Dimer (D2D) in plasma. |
Pig D-Dimer (D2D) ELISA Kit |
CEA506Po-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3323.45 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Pig D-Dimer (D2D) in plasma. |
Pig D-Dimer (D2D) ELISA Kit |
4-CEA506Po |
Cloud-Clone |
-
EUR 6178.80
-
EUR 3264.00
-
EUR 819.60
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Pig D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species. |
Rat D-Dimer (D2D) ELISA Kit |
CEA506Ra-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5552.14 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat D-Dimer (D2D) in plasma. |
Rat D-Dimer (D2D) ELISA Kit |
CEA506Ra-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 562.42 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat D-Dimer (D2D) in plasma. |
Rat D-Dimer (D2D) ELISA Kit |
CEA506Ra-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 752.02 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat D-Dimer (D2D) in plasma. |
Rat D-Dimer (D2D) ELISA Kit |
CEA506Ra-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3024.07 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat D-Dimer (D2D) in plasma. |
Rat D-Dimer (D2D) ELISA Kit |
4-CEA506Ra |
Cloud-Clone |
-
EUR 5612.40
-
EUR 2965.20
-
EUR 752.40
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rat D-Dimer (D2D) in samples from plasma with no significant corss-reactivity with analogues from other species. |
Rabbit D-Dimer (D2D) ELISA Kit |
CEA506Rb-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5552.14 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit D-Dimer (D2D) in Plasma. |
Rabbit D-Dimer (D2D) ELISA Kit |
CEA506Rb-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 562.42 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit D-Dimer (D2D) in Plasma. |
Rabbit D-Dimer (D2D) ELISA Kit |
CEA506Rb-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 752.02 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit D-Dimer (D2D) in Plasma. |
Rabbit D-Dimer (D2D) ELISA Kit |
CEA506Rb-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3024.07 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit D-Dimer (D2D) in Plasma. |
Rabbit D-Dimer (D2D) ELISA Kit |
4-CEA506Rb |
Cloud-Clone |
-
EUR 5612.40
-
EUR 2965.20
-
EUR 752.40
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rabbit D-Dimer (D2D) in samples from Plasma with no significant corss-reactivity with analogues from other species. |
Rabbit D-Dimer,D2D ELISA Kit |
CN-00585R1 |
ChemNorm |
96T |
EUR 546 |
This exhibits the potential of canine in sustaining and spreading the an infection in Malaysia. As a result of occupational threat on account of frequent contact with canine and publicity to contaminated environments, canine handlers needs to be made conscious of the presence of this zoonotic illness.
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